Recombinant human NLK protein
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Recombinant human NLK protein is a Human Full Length protein, in the 1 to 527 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, WB, FuncS.
View Alternative Names
LAK1, NLK, Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1
- FuncS
Unknown
Functional Studies - Recombinant human NLK protein (AB107700)
Specific activity of ab107700 was determined to be 2.4 nmol/min/mg.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human NLK protein (AB107700)
SDS-PAGE analysis of ab107700
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NLK participates in regulating cellular processes such as cell cycle apoptosis and transcription. It is not part of a larger complex but works within signaling pathways to influence these processes. NLK modulates the activity of several transcription factors particularly in the Wnt/beta-catenin signaling pathway where it negatively regulates the signaling to control gene expression relevant to cell proliferation and differentiation.
Pathways
NLK is an important player in the Wnt/beta-catenin and MAPK pathways. In the Wnt/beta-catenin pathway it associates with proteins such as TCF/LEF and beta-catenin acting to modulate transcription by phosphorylating these molecules. The MAPK pathway involves NLK in response to stress signals coordinating with proteins like TAK1 to mediate cellular responses. These pathways illustrate NLK's role in transmitting signals that govern cell fate and stress responses.
Specifications
Form
Liquid
Additional notes
The purity was determined to be >85% by densitometry.GST Affinity Column purified.
General info
Function
Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination (PubMed : 12482967, PubMed : 14960582, PubMed : 15004007, PubMed : 15764709, PubMed : 20061393, PubMed : 20874444, PubMed : 21454679). Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2 (PubMed : 15004007, PubMed : 15764709). Negative regulator of the canonical Wnt/beta-catenin signaling pathway (PubMed : 12482967). Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1 (PubMed : 21454679). Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes (PubMed : 12482967, PubMed : 21454679). Negative regulator of the Notch signaling pathway (PubMed : 20118921). Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1 (PubMed : 20118921). Negative regulator of the MYB family of transcription factors (PubMed : 15082531). Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP (PubMed : 15082531). Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself (PubMed : 15082531). Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 (PubMed : 15004007, PubMed : 15764709). Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members (PubMed : 25512613). Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed : 25512613). Acts as an inhibitor of the mTORC1 complex in response to osmotic stress by mediating phosphorylation of RPTOR, thereby preventing recruitment of the mTORC1 complex to lysosomes (PubMed : 26588989).
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Post-translational modifications
Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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