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AB271635

Recombinant Human NLRP3 + NEK7 protein (7x His tag N-Term, DDDDK tag N-Term + Avi tag N-Term)

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Recombinant Human NLRP3 + NEK7 protein (7x His tag N-Term, DDDDK tag N-Term + Avi tag N-Term) is a Human Full Length protein, in the 2 to 302 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE.

View Alternative Names

Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7, C1orf7, CIAS1, NALP3, PYPAF1, NLRP3, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, Cold-induced autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, CLR1.1

1 Images
SDS-PAGE - Recombinant Human NLRP3 + NEK7 protein (7x His tag N-Term, DDDDK tag N-Term + Avi tag N-Term) (AB271635)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NLRP3 + NEK7 protein (7x His tag N-Term, DDDDK tag N-Term + Avi tag N-Term) (AB271635)

SDS-PAGE analysis of ab271635.

Key facts

Purity

>90% SDS-PAGE

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8TDX7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Proteins & Peptides

AB320811

Recombinant Mouse NLRP3 Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse NLRP3 Protein Standard (His tag) (AB320811)

  • 0
  • 0
Primary Antibodies

AB133514

Anti-NEK7 antibody [EPR4900]

RabMAb|Recombinant|KO Validated|Trial Size

Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (AB133514)

  • 4
  • 2
Primary Antibodies

AB263899

Anti-NLRP3 antibody [EPR23094-1]

KO Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-NLRP3 antibody [EPR23094-1] (AB263899)

  • 4
  • 2
Primary Antibodies

AB179515

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

  • 3
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"DEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":302,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q8TDX7","tags":[{"tag":"7x His","terminus":"N-Terminus"}]},{"sequence":"KDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1036,"aminoAcidStart":134,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q96P20","tags":[{"tag":"DDDDK","terminus":"N-Terminus"},{"tag":"Avi","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NLRP3 protein also known as NACHT LRR and PYD domains-containing protein 3 and NEK7 known as NIMA-related kinase 7 play integral parts in cellular pathways. NLRP3 has a molecular mass of approximately 118 kDa and is expressed mainly in innate immune cells such as macrophages and dendritic cells. NEK7 with a mass around 34 kDa is largely expressed in dividing cells and performs important cellular roles during the cell cycle. These proteins are part of the NLRP3 inflammasome complex a critical component of the immune response which detects pathogenic microorganisms and stress signals.
Biological function summary

NLRP3 and NEK7 are involved in innate immunity playing key roles in inflammation. The NLRP3 inflammasome comprises NLRP3 ASC (apoptosis-associated speck-like protein containing a CARD) and pro-caspase-1. NEK7 acts as a positive regulator of NLRP3 inflammasome activation. This complex once formed leads to the activation of caspase-1 which then processes pro-inflammatory cytokines like IL-1β and IL-18 into their active forms. This process aids in the defense against infections but its dysregulation can result in excessive inflammation.

Pathways

NLRP3 and NEK7 are instrumental in the inflammasome and IL-1 signaling pathways. Within the inflammasome signaling NLRP3 acts as a pattern recognition receptor and its interaction with NEK7 is critical for sensing cell danger signals. This recognition and activation also involve other proteins such as ASC linking the inflammasome pathway to wider immune responses. The IL-1 signaling pathway further connects NLRP3 inflammasome activity to downstream effects promoting inflammatory responses and influencing adaptive immunity.

NLRP3 and NEK7 are linked to autoinflammatory diseases and neurodegenerative disorders. One well-studied association is with Cryopyrin-associated periodic syndromes (CAPS) where mutations in NLRP3 lead to abnormal inflammasome activation causing chronic inflammation. Another connection is identified in Alzheimer’s disease where the pathologic activation of the NLRP3 inflammasome contributes to neuroinflammation. Proteins such as ASC and caspase-1 are also implicated in these conditions highlighting the potential of targeting the inflammasome for therapeutic strategies.
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Specifications

Form

Liquid

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General info

Function

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed : 17101132, PubMed : 19941817, PubMed : 31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed : 17586473, PubMed : 19414596, PubMed : 19941817, PubMed : 26522158, PubMed : 31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed : 31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed : 26642356, PubMed : 36442502, PubMed : 39173637). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3 : PYCARD complex, and activation of CASP1 (PubMed : 26642356, PubMed : 31189953, PubMed : 36442502, PubMed : 39173637, PubMed : 40450990). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division : interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed : 26642356, PubMed : 31189953).

Sequence similarities

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Post-translational modifications

Phosphorylation at Ser-195 required for its activation.

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Product protocols

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Target data

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed : 17101132, PubMed : 19941817, PubMed : 31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed : 17586473, PubMed : 19414596, PubMed : 19941817, PubMed : 26522158, PubMed : 31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed : 31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed : 26642356, PubMed : 36442502, PubMed : 39173637). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3 : PYCARD complex, and activation of CASP1 (PubMed : 26642356, PubMed : 31189953, PubMed : 36442502, PubMed : 39173637, PubMed : 40450990). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division : interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed : 26642356, PubMed : 31189953).
See full target information NEK7

Additional targets

NLRP3
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