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AB157274

Recombinant human NMNAT3 protein

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Recombinant human NMNAT3 protein is a Human Full Length protein, in the 1 to 252 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

FKSG76, NMNAT3, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3, NMN/NaMN adenylyltransferase 3, Nicotinamide-nucleotide adenylyltransferase 3, Nicotinate-nucleotide adenylyltransferase 3, Pyridine nucleotide adenylyltransferase 3, NMN adenylyltransferase 3, NaMN adenylyltransferase 3, PNAT-3

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 2.35 U/mg.

Accession

Q96T66

Animal free

No

Carrier free

No

Species

Human

Storage buffer

Constituents: 10% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.79% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MKSRIPVVLLACGSFNPITNMHLRMFEVARDHLHQTGMYQVIQGIISPVNDTYGKKDLAASHHRVAMARLALQTSDWIRVDPWESEQAQWMETVKVLRHHHSKLLRSPPQMEGPDHGKALFSTPAAVPELKLLCGADVLKTFQTPNLWKDAHIQEIVEKFGLVCVGRVGHDPKGYIAESPILRMHQHNIHLAKEPVQNEISATYIRRALGQGQSVKYLIPDAVITYIKDHGLYTKGSTWKGKSTQSTEGKTS","proteinLength":"Full Length","predictedMolecularWeight":"30.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":252,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96T66","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMNAT3 or nicotinamide nucleotide adenylyltransferase 3 catalyzes the biosynthesis of NAD+ from NMN and ATP playing a role in cellular energy metabolism. Alternate names include NMNAT3p and Enzyme NIC. It has a molecular mass of around 31 kDa. NMNAT3 is expressed in a variety of tissues with significant expression in red blood cells and the mitochondria of other cell types.
Biological function summary

NMNAT3 participates in the NAD+ salvage pathway transforming NMN into NAD+ a fundamental coenzyme in cellular metabolism and redox reactions. It does not form part of any larger complex but functions independently within the cell. Its activity supports energy production DNA repair and cell signaling processes making it an important player in maintaining cellular homeostasis.

Pathways

The salvage pathway for NAD+ biosynthesis relies heavily on NMNAT3's enzymatic action. It works alongside related proteins like NMNAT1 and NMNAT2 which catalyze similar reactions in different cellular locations. This pathway intersects with the oxidative phosphorylation process a critical energy-producing pathway within mitochondria.

NMNAT3 has implications in conditions such as anemia and mitochondrial dysfunction. Altered expression or mutation of NMNAT3 may contribute to anemia by affecting red blood cell metabolism and NAD+ homeostasis. The protein also relates to neurodegenerative disorders due to its role in mitochondrial function and energy balance aligning with proteins like Sirtuins which also regulate NAD+ levels and have implications in cellular aging processes.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following injury.

Sequence similarities

Belongs to the eukaryotic NMN adenylyltransferase family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following injury.
See full target information NMNAT3
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