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AB128450

Recombinant Human NMRAL1 protein

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Recombinant Human NMRAL1 protein is a Human Full Length protein, in the 1 to 299 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

HSCARG, NMRAL1, NmrA-like family domain-containing protein 1

1 Images
SDS-PAGE - Recombinant Human NMRAL1 protein (AB128450)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NMRAL1 protein (AB128450)

15% SDS-PAGE showing ab128450 at approximately 35.9 kDa (3µg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9HBL8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Not currently tested for endotoxin levels
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMVDKKLVVVFGGTGAQGGSVARTLLEDGTFKVRVVTRNPRKKAAKELRLQGAEVVQGDQDDQVIMELALNGAYATFIVTNYWESCSQEQEVKQGKLLADLARRLGLHYVVYSGLENIKKLTAGRLAAAHFDGKGEVEEYFRDIGVPMTSVRLPCYFENLLSHFLPQKAPDGKSYLLSLPTGDVPMDGMSVSDLGPVVLSLLKMPEKYVGQNIGLSTCRHTAEEYAALLTKHTRKVVHDAKMTPEDYEKLGFPGARDLANMFRFYALRPDRDIELTLRLNPKALTLDQWLEQHKGDFNLL","proteinLength":"Full Length","predictedMolecularWeight":"35.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":299,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9HBL8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMRAL1 also known as Nuclear receptor coactivator 5 (Ncoa5) is a protein involved in cellular response to oxidative stress. The encoded protein weighing approximately 42 kDa localizes to the cytoplasm and nucleus and shows widespread expression in various tissues. Researchers often study NMRAL1 because of its potential interactions with oxidized nicotinamide adenine dinucleotide (NAD+) levels affecting cellular responses.
Biological function summary

The NMRAL1 protein assists in regulating oxidative stress responses and energy metabolism in cells. This protein may form part of multiprotein complexes that aid in transcriptional regulation by interacting with other transcription factors. It modulates cellular antioxidant responses indirectly influencing gene expression related to stress and metabolism. Scientists observe its high expression in cells facing oxidative challenges suggesting a role in protecting cellular integrity.

Pathways

NMRAL1 has key functions in energy metabolism and redox signaling. It fits into the oxidative stress response pathway working alongside Nrf2 a critical transcription factor that regulates antioxidant proteins. The involvement of NMRAL1 in modulating redox balance highlights its interconnection with proteins like SIRT1 which also regulate cellular metabolism and oxidative stress.

NMRAL1 shows connections to metabolic diseases such as diabetes where oxidative stress plays a significant role. The protein also links to cancer where it affects tumor development through modulation of oxidative stress pathways. NMRAL1 interacts indirectly with proteins like p53 in cancer influencing cell cycle regulation and survival in response to oxidative damage.
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Specifications

Form

Liquid

Additional notes

ab128450 is purified using conventional chromatography techniques (anion exchange and gel filtration)

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General info

Function

Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer.

Sequence similarities

Belongs to the NmrA-type oxidoreductase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer.
See full target information NMRAL1
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Complementary Products

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AB272637

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMRAL1 antibody (AB272637)

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Primary Antibodies

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Primary Antibodies

AB315032

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Product promise

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