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AB202220

Recombinant Human NMT1/NMT protein (His tag N-Terminus)

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Recombinant Human NMT1/NMT protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 496 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

NMT, NMT1, Glycylpeptide N-tetradecanoyltransferase 1, Myristoyl-CoA:protein N-myristoyltransferase 1, Peptide N-myristoyltransferase 1, Protein-lysine myristoyltransferase NMT1, HsNMT1, NMT 1, Type I N-myristoyltransferase

1 Images
SDS-PAGE - Recombinant Human NMT1/NMT protein (AB202220)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NMT1/NMT protein (AB202220)

SDS-PAGE analysis of Recombinant Human NMT1/NMT protein (ab202220), under reducing conditions. Proteins visualized by coomassie blue stain.

Lane 1 : Molecular Weight Standards

Lane 2 : 3 μg Recombinant Human NMT1/NMT protein

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P30419

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as NMT1
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMADESETAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKKKQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQFWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDTEKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWHRSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLTRYLKQFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKAAYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWKCPSMGAEKVGLVLQ","proteinLength":"Full Length","predictedMolecularWeight":"59.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":496,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30419","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-myristoyltransferase 1 often referred to as NMT1 or NMT is an enzyme essential for catalyzing the transfer of myristate a 14-carbon saturated fatty acid to the N-terminal glycine residue of substrate proteins. This post-translational modification is called myristoylation and is necessary for the proper functioning of many proteins. NMT1 has a molecular mass of approximately 50 kDa and is widely expressed in eukaryotic cells prominently in the cytoplasm and sometimes associated with intracellular membranes.
Biological function summary

NMT1 modifies proteins by attaching myristic acid influencing their membrane-binding capability stability and function. It does not form part of a larger complex but rather acts individually to regulate protein activity through myristoylation. This modification is important for the localization and activation of proteins involved in various cellular processes including signal transduction and apoptosis.

Pathways

N-myristoyltransferase 1 plays a role in several key biological pathways. Myristoylation mediated by NMT1 regulates signal transduction pathways such as the Src family kinases and G-protein signaling. Through these pathways NMT1 interacts with key proteins like Src a tyrosine kinase involved in cell growth and differentiation and Gα proteins which are part of the G-protein coupled receptor signaling pathway.

Alterations in NMT1 activity are associated with various cancers and infectious diseases. For instance overexpression of NMT1 has been observed in colorectal cancer potentially linked through its interactions with cancer-related proteins like Src. Additionally the enzyme is important for the survival of certain pathogens which require myristoylation for their life cycle suggesting that NMT1 could be a target for antimicrobial drug development.
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Specifications

Form

Liquid

Additional notes

ab202220 was purified using conventional chromatography techniques.

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General info

Function

Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins (PubMed : 22865860, PubMed : 25255805, PubMed : 32686708, PubMed : 34999170, PubMed : 9353336, PubMed : 9506952). Also able to mediate N-terminal lysine myristoylation of proteins : catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3' (PubMed : 32103017, PubMed : 32111831). Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle (PubMed : 32103017).

Sequence similarities

Belongs to the NMT family.

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Product protocols

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Target data

Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins (PubMed : 22865860, PubMed : 25255805, PubMed : 32686708, PubMed : 34999170, PubMed : 9353336, PubMed : 9506952). Also able to mediate N-terminal lysine myristoylation of proteins : catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3' (PubMed : 32103017, PubMed : 32111831). Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle (PubMed : 32103017).
See full target information NMT1
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