Skip to main content

Recombinant human NNMT protein (Active) is a Human Full Length protein, in the 1 to 264 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE, FuncS.

Be the first to review this product! Submit a review

Images

SDS-PAGE - Recombinant human NNMT protein (Active) (AB271638), expandable thumbnail
  • Functional Studies - Recombinant human NNMT protein (Active) (AB271638), expandable thumbnail

Key facts

Purity
>=90% SDS-PAGE
Expression system
HEK 293 cells
Tags
His tag C-Terminus
Applications
SDS-PAGE, FuncS
Biologically active
Yes

Amino acid sequence

M E S G F T S K D T Y L S H F N P R D Y L E K Y Y K F G S R H S A E S Q I L K H L L K N L F K I F C L D G V K G D L L I D I G S G P T I Y Q L L S A C E S F K E I V V T D Y S D Q N L Q E L E K W L K K E P E A F D W S P V V T Y V C D L E G N R V K G P E K E E K L R Q A V K Q V L K C D V T Q S Q P L G A V P L P P A D C V L S T L C L D A A C P D L P T Y C R A L R N L G S L L K P G G F L V I M D A L K S S Y Y M I G E Q K F S S L P L G R E A V E A A V K E A G Y T I E W F E V I S Q S Y S S T M A N N E G L F S L V A R K L S R P L

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PubMed:21823666, PubMed:23455543, PubMed:8182091). Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PubMed:23455543, PubMed:26571212, PubMed:31043742). In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state (PubMed:26571212). Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD(+) levels through the salvage pathway (By similarity). Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase (By similarity). Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification (PubMed:30044909).

Alternative names

Recommended products

Recombinant human NNMT protein (Active) is a Human Full Length protein, in the 1 to 264 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity
>=90% SDS-PAGE
Expression system
HEK 293 cells
Applications
SDS-PAGE, FuncS
Biological activity
Specific activity: 57 pmol/min/μg

Assay Conditions: 10 mM Tris-HCl, pH 7.4, 0.05% Tween 20, 1 mM DTT, 100 μM Quinoline, 100 μM SAM, 30 min, room temperature

Accession
P40261-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Preservative: 0.99% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

Sequence info

Amino acid sequence

M E S G F T S K D T Y L S H F N P R D Y L E K Y Y K F G S R H S A E S Q I L K H L L K N L F K I F C L D G V K G D L L I D I G S G P T I Y Q L L S A C E S F K E I V V T D Y S D Q N L Q E L E K W L K K E P E A F D W S P V V T Y V C D L E G N R V K G P E K E E K L R Q A V K Q V L K C D V T Q S Q P L G A V P L P P A D C V L S T L C L D A A C P D L P T Y C R A L R N L G S L L K P G G F L V I M D A L K S S Y Y M I G E Q K F S S L P L G R E A V E A A V K E A G Y T I E W F E V I S Q S Y S S T M A N N E G L F S L V A R K L S R P L
Accession
P40261
Protein length
Full Length
Predicted molecular weight
30 kDa
Amino acids
1 to 264
Nature
Recombinant
Tags
His tag C-Terminus

Specifications

Form
Liquid

General info

Function

Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PubMed:21823666, PubMed:23455543, PubMed:8182091). Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PubMed:23455543, PubMed:26571212, PubMed:31043742). In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state (PubMed:26571212). Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD(+) levels through the salvage pathway (By similarity). Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase (By similarity). Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification (PubMed:30044909).

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.

Post-translational modifications

Deiminated by PADI1 and PADI2.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The NNMT protein also known as Nicotinamide N-methyltransferase acts by catalyzing the methylation of nicotinamide using S-adenosylmethionine as a methyl donor. This enzyme has a mass of around 29 kDa and is expressed in various tissues including liver adipose tissue and certain cancer cells. The NNMT gene provides instructions for creating the protein that plays significant roles in metabolic processes and cellular homeostasis.

Biological function summary

NNMT impacts cell energy balance and gene expression regulation by influencing the levels of nicotinamide and methionine metabolites. This enzyme does not typically form part of a complex acting more so as an individual regulator of methylation reactions affecting cellular metabolism. Researchers find interest in NNMT for its role in modulating cellular growth and proliferation forming a link to both normal cellular functions and pathological conditions like cancer.

Pathways

NNMT has a significance in NAD+ metabolism and methionine salvage pathways. These pathways are essential in maintaining redox homeostasis and proper methylation status in cells. NNMT through its enzymatic activity interacts with SAM (S-adenosyl methionine) closely relating its function to methylation processes that involve other proteins such as SIRTs (Sirtuins) and NAMPT (Nicotinamide phosphoribosyltransferase) within these metabolic pathways.

Associated diseases and disorders

NNMT shows relevance in cancer and obesity-related complications. The protein's upregulation in cancer types like breast and colorectal cancers links it to tumor development and progression. In obesity NNMT's involvement in adipose tissue might contribute to metabolic dysregulation. The enzyme also interconnects with proteins like insulin receptors and adiponectin highlighting its potential role in metabolic diseases and making it an interesting target for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Recombinant human NNMT protein (Active) (ab271638), expandable thumbnail

    SDS-PAGE - Recombinant human NNMT protein (Active) (ab271638)

    SDS-PAGE analysis of ab271638.

  • Functional Studies - Recombinant human NNMT protein (Active) (ab271638), expandable thumbnail

    Functional Studies - Recombinant human NNMT protein (Active) (ab271638)

    Specifc activity of ab271638 was 57 pmol/min/μg.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com