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AB111634

Recombinant Human NNT1 protein (denatured)

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Recombinant Human NNT1 protein (denatured) is a Human Full Length protein, in the 28 to 225 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

BSF3, CLC, NNT1, CLCF1, Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3, Novel neurotrophin-1, BSF-3, NNT-1

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SDS-PAGE - Recombinant Human NNT1 protein (denatured) (AB111634)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NNT1 protein (denatured) (AB111634)

15% SDS-PAGE showing ab111634 at approximately 24.6kDa (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9UBD9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 3.5 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.59% Sodium citrate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMLNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVDLEVWRSLNDKLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMAALGYPLPQPLPGTEPTWTPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAAAVTLHLGAHGF","proteinLength":"Full Length","predictedMolecularWeight":"24.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":225,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UBD9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein 'NNT1' also known as nicotinamide nucleotide transhydrogenase is a mitochondrial enzyme with a mass of approximately 114 kDa. It is expressed in various tissues with high concentrations in the heart liver and kidney. Mechanically NNT1 is a membrane-bound enzyme that acts to transfer hydride ions between NADH and NADP+ facilitating the production of NADPH within the mitochondria. This process is important for maintaining cellular redox balance and supporting antioxidant defense systems.
Biological function summary

The function of maintaining NADPH levels makes NNT1 an important player in protecting cells from oxidative damage. It functions as part of the inner mitochondrial membrane and is not generally considered to be part of a larger complex in its basic form. By perpetuating a pool of available NADPH NNT1 aids cellular processes such as lipid synthesis and detoxification.

Pathways

The activity of NNT1 is integral to the maintenance of mitochondrial homeostasis particularly the oxidative phosphorylation pathway. It has associations with the glutathione antioxidant pathway as it contributes to the regeneration of reduced glutathione through provision of NADPH. The pathways involving NNT1 connect it to proteins such as glutathione reductase and those involved in the electron transport chain.

NNT1 has connections to metabolic disorders and conditions related to oxidative stress. Mutations in the NNT1 gene can be linked to familial glucocorticoid deficiency a condition affecting adrenal gland function. This protein is also related to oxidative stress which can connect it to neurodegenerative disorders with ties to proteins involved in mitochondrial dysfunction and stress responses.
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Specifications

Form

Liquid

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General info

Function

In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development (Probable). Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.

Sequence similarities

Belongs to the IL-6 superfamily.

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Product protocols

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Target data

In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development (Probable). Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.
See full target information CLCF1
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Complementary Products

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AB251886

Anti-NNT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNT1 antibody (AB251886)

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

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Primary Antibodies

AB18207

Anti-beta III Tubulin antibody - Neuronal Marker

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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB18207)

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