Recombinant human Noggin protein (Active)
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(1 Publication)
Recombinant human Noggin protein (Active) is a Human Full Length protein, in the 28 to 232 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.
View Alternative Names
Noggin, NOG
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant human Noggin protein (Active) (AB281817)
Mass determination by ESI-TOF.
Predicted MW is 23104.64 Da. (+/- 10 Da by ESI-TOF). Observes MW is 23106.53 Da. Additional masses at 23382.04 is due to residual O-glycans.
- FuncS
Supplier Data
Functional Studies - Recombinant human Noggin protein (Active) (AB281817)
Functional analysis of ab281817.
Fully biologically active determined by its ability to inhibit BMP-4 induced alkaline phosphatase activity in ATDC5 cells.
ED50 for this effect is ≤65.37 ng/ml, corresponding to a specific activity of 1.52 x 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot : GR3387043-1
- HPLC
Supplier Data
HPLC - Recombinant human Noggin protein (Active) (AB281817)
HPLC analysis of ab281817.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human Noggin protein (Active) (AB281817)
SDS-PAGE analysis of ab281817.
Complementary Products
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Noggin functions as an essential regulator in embryonic development sculpting tissues by inhibiting BMP activity. It does not work alone but functions as part of a complex regulatory network involving other BMP antagonists. By inhibiting BMPs Noggin plays an important role in neural tube closure limb formation and somite development. Noggin's activities help modulate the balance between proliferation and differentiation in these contexts allowing for proper structural formation during embryogenesis.
Pathways
Noggin operates mainly within the TGF-beta/BMP signaling pathway serving as a critical modulator. Inhibiting BMP through Noggin affects downstream targets connecting it to other signaling proteins like SMADs which mediate transcriptional responses. This control enables Noggin to indirectly regulate Wnt signaling as well showing its impact on various developmental stages and processes. Its influence on these pathways indicates Noggin's importance in maintaining cellular homeostasis during growth and morphogenesis.
Specifications
Form
Lyophilized
Additional notes
>=95% Purity by HPLC
General info
Function
Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed : 21976273, PubMed : 26643732).
Sequence similarities
Belongs to the noggin family.
Target data
Publications (1)
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BMC cancer 23:594 PubMed37370018
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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