Recombinant human Noggin protein (Active) is a Human Full Length protein, in the 28 to 232 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
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Publications
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- BMC cancer 23:5942023High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBingwei Ma,Xingchun Wang,Hui Ren,Yingying Li,Haijiao Zhang,Muqing Yang,Jiyu LiPubMed 37370018
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
Q H Y L H I R P A P S D N L P L V D L I E H P D P I F D P K E K D L N E T L L R S L L G G H Y D P G F M A T S P P E D R P G G G G G A A G G A E D L A E L D Q L L R Q R P S G A M P S E I K G L E F S E G L A Q G K K Q R L S K K L R R K L Q M W L W S Q T F C P V L Y A W N D L G S R F W P R Y V K V G S C F S K R S C S V P E G M V C K P S K S V H L T V L R W R C Q R R G G Q R C G W I P I Q Y P I I S E C K C S C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed:21976273, PubMed:26643732).
Alternative names
Noggin, NOG
Recommended products
Recombinant human Noggin protein (Active) is a Human Full Length protein, in the 28 to 232 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by its ability to inhibit BMP-4 induced alkaline phosphatase activity in ATDC5 cells.
ED50 for this effect is ≤65.37 ng/ml, corresponding to a specific activity of 1.52 x 104 units/mg.
- Accession
- Q13253-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Q H Y L H I R P A P S D N L P L V D L I E H P D P I F D P K E K D L N E T L L R S L L G G H Y D P G F M A T S P P E D R P G G G G G A A G G A E D L A E L D Q L L R Q R P S G A M P S E I K G L E F S E G L A Q G K K Q R L S K K L R R K L Q M W L W S Q T F C P V L Y A W N D L G S R F W P R Y V K V G S C F S K R S C S V P E G M V C K P S K S V H L T V L R W R C Q R R G G Q R C G W I P I Q Y P I I S E C K C S C
- Accession
- Q13253
- Protein length
- Full Length
- Predicted molecular weight
- 23.1 kDa
- Actual molecular weight
- 23.11 kDa
- Amino acids
- 28 to 232
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>=95% Purity by HPLC
General info
- Function
Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed:21976273, PubMed:26643732).
- Sequence similarities
Belongs to the noggin family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Noggin also known as noggin protein is a secreted glycoprotein with a mass of approximately 26 kDa. It antagonizes bone morphogenetic proteins (BMPs) by directly binding them preventing their interaction with receptors. This binding inhibits the signaling pathway which affects cellular processes such as differentiation growth and apoptosis. Noggin expresses prominently in embryonic tissues and in tissues undergoing differentiation particularly in the neural tube notochord and developing articular cartilage. The expression pattern suggests its importance during critical phases of development and tissue patterning.
- Biological function summary
Noggin functions as an essential regulator in embryonic development sculpting tissues by inhibiting BMP activity. It does not work alone but functions as part of a complex regulatory network involving other BMP antagonists. By inhibiting BMPs Noggin plays an important role in neural tube closure limb formation and somite development. Noggin's activities help modulate the balance between proliferation and differentiation in these contexts allowing for proper structural formation during embryogenesis.
- Pathways
Noggin operates mainly within the TGF-beta/BMP signaling pathway serving as a critical modulator. Inhibiting BMP through Noggin affects downstream targets connecting it to other signaling proteins like SMADs which mediate transcriptional responses. This control enables Noggin to indirectly regulate Wnt signaling as well showing its impact on various developmental stages and processes. Its influence on these pathways indicates Noggin’s importance in maintaining cellular homeostasis during growth and morphogenesis.
- Associated diseases and disorders
Noggin relates to conditions such as fibrodysplasia ossificans progressiva and cartilage-related abnormalities. Mutations or dysregulation of noggin protein can disrupt normal BMP signaling leading to aberrant skeletal development and calcification. This disruption often involves interactions with other proteins like BMP4 revealing Noggin's critical role in maintaining bone and cartilage integrity. Targeting noggin pathway components may provide therapeutic potential for addressing such conditions.
Product promise
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Terms & Conditions.
4 product images
Functional Studies - Recombinant human Noggin protein (Active) (ab281817)
Functional analysis of ab281817.
Fully biologically active determined by its ability to inhibit BMP-4 induced alkaline phosphatase activity in ATDC5 cells.
ED50 for this effect is ≤65.37 ng/ml, corresponding to a specific activity of 1.52 x 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3387043-1
SDS-PAGE - Recombinant human Noggin protein (Active) (ab281817)
SDS-PAGE analysis of ab281817.
Mass Spectrometry - Recombinant human Noggin protein (Active) (ab281817)
Mass determination by ESI-TOF.
Predicted MW is 23104.64 Da. (+/- 10 Da by ESI-TOF). Observes MW is 23106.53 Da. Additional masses at 23382.04 is due to residual O-glycans.
HPLC - Recombinant human Noggin protein (Active) (ab281817)
HPLC analysis of ab281817.
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