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Recombinant Human Notch2 protein is a Human Fragment protein, in the 27 to 136 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Alternative names=Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Sequence
Q C R D G Y E P C V N E G M C V T Y H N G T G Y C K C P E G F L G E Y C Q H R D P C E K N R C Q N G G T C V A Q A M L G K A T C R C A S G F T G E D C Q Y S T S H P C F V S R P C L N G G T C H M L S R D T Y E C T C Q V G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Recombinant protein |
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Recombinant Human Notch2 protein is a Human Fragment protein, in the 27 to 136 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Alternative names=Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2
Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Predicted molecular weight
37.73 kDa
- Amino acids
27 to 136
- Sequence
Q C R D G Y E P C V N E G M C V T Y H N G T G Y C K C P E G F L G E Y C Q H R D P C E K N R C Q N G G T C V A Q A M L G K A T C R C A S G F T G E D C Q Y S T S H P C F V S R P C L N G G T C H M L S R D T Y E C T C Q V G
- Accession
- Q04721-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Specifications
- Form
Liquid
General info
- Function
Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed:21378985, PubMed:21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:29149593). Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
- Sequence similarities
Belongs to the NOTCH family.
- Post-translational modifications
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form (By similarity). Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC) (By similarity). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT) (By similarity). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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