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Recombinant Human Notch2 protein (His tag) is a Human Fragment protein, in the 1 to 530 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human Notch2 protein (His tag) (AB276341), expandable thumbnail

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
Baculovirus infected insect cells
Tags
His tag C-Terminus
Applications
SDS-PAGE
Biologically active
No

Amino acid sequence

M P A L R P A L L W A L L A L W L C C A A P A H A L Q C R D G Y E P C V N E G M C V T Y H N G T G Y C K C P E G F L G E Y C Q H R D P C E K N R C Q N G G T C V A Q A M L G K A T C R C A S G F T G E D C Q Y S T S H P C F V S R P C L N G G T C H M L S R D T Y E C T C Q V G F T G K E C Q W T D A C L S H P C A N G S T C T T V A N Q F S C K C L T G F T G Q K C E T D V N E C D I P G H C Q H G G T C L N L P G S Y Q C Q C P Q G F T G Q Y C D S L Y V P C A P S P C V N G G T C R Q T G D F T F E C N C L P G F E G S T C E R N I D D C P N H R C Q N G G V C V D G V N T Y N C R C P P Q W T G Q F C T E D V D E C L L Q P N A C Q N G G T C A N R N G G Y G C V C V N G W S G D D C S E N I D D C A F A S C T P G S T C I D R V A S F S C M C P E G K A G L L C H L D D A C I S N P C H K G A L C D T N P L N G Q Y I C T C P Q G Y K G A D C T E D V D E C A M A N S N P C E H A G K C V N T D G A F H C E C L K G Y A G P R C E M D I N E C H S D P C Q N D A T C L D K I G G F T C L C M P G F K G V H C E L E I N E C Q S N P C V N N G Q C V D K V N R F Q C L C P P G F T G P V C Q

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed:21378985, PubMed:21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:29149593). Positively regulates self-renewal of liver cancer cells (PubMed:25985737).

Alternative names

Recommended products

Recombinant Human Notch2 protein (His tag) is a Human Fragment protein, in the 1 to 530 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
Baculovirus infected insect cells
Applications
SDS-PAGE
Accession
Q04721-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.9% Sodium chloride, 0.24% Tris

Sequence info

Amino acid sequence

M P A L R P A L L W A L L A L W L C C A A P A H A L Q C R D G Y E P C V N E G M C V T Y H N G T G Y C K C P E G F L G E Y C Q H R D P C E K N R C Q N G G T C V A Q A M L G K A T C R C A S G F T G E D C Q Y S T S H P C F V S R P C L N G G T C H M L S R D T Y E C T C Q V G F T G K E C Q W T D A C L S H P C A N G S T C T T V A N Q F S C K C L T G F T G Q K C E T D V N E C D I P G H C Q H G G T C L N L P G S Y Q C Q C P Q G F T G Q Y C D S L Y V P C A P S P C V N G G T C R Q T G D F T F E C N C L P G F E G S T C E R N I D D C P N H R C Q N G G V C V D G V N T Y N C R C P P Q W T G Q F C T E D V D E C L L Q P N A C Q N G G T C A N R N G G Y G C V C V N G W S G D D C S E N I D D C A F A S C T P G S T C I D R V A S F S C M C P E G K A G L L C H L D D A C I S N P C H K G A L C D T N P L N G Q Y I C T C P Q G Y K G A D C T E D V D E C A M A N S N P C E H A G K C V N T D G A F H C E C L K G Y A G P R C E M D I N E C H S D P C Q N D A T C L D K I G G F T C L C M P G F K G V H C E L E I N E C Q S N P C V N N G Q C V D K V N R F Q C L C P P G F T G P V C Q
Accession
Q04721
Protein length
Fragment
Predicted molecular weight
55.5 kDa
Amino acids
1 to 530
Nature
Recombinant
Tags
His tag C-Terminus

Specifications

Form
Lyophilized

General info

Function

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed:21378985, PubMed:21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:29149593). Positively regulates self-renewal of liver cancer cells (PubMed:25985737).

Sequence similarities

Belongs to the NOTCH family.

Post-translational modifications

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form (By similarity). Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC) (By similarity). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT) (By similarity). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).

Subcellular localisation
Nucleus

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Notch2 also known as neurogenic locus notch homolog protein 2 is a transmembrane receptor protein that plays a critical role in cell differentiation and development. It is part of the Notch signaling pathway which is highly conserved across species. The protein weighs approximately 270 kilodaltons and is primarily found in the kidney liver and various other tissues. Notch2 activation occurs through interactions with specific ligands leading to the release of its intracellular domain which enters the nucleus to regulate gene expression.

Biological function summary

Notch2 modulates important developmental processes including lineage specification and cell fate determination. This protein is vital in maintaining the homeostasis of several organs and tissues and it does this by being part of the Notch signaling complex. Additionally Notch2 contributes to hematopoiesis influencing the balance between different blood cell types. Researchers have also noted its role in maintaining stem cell populations and facilitating the proper function of the immune system.

Pathways

Notch2 integrates into the canonical Notch signaling pathway which impacts cellular communication and gene transcription. This pathway interlinks with the Wnt signaling pathway influencing tissue regeneration and cancer development. Notch2 itself associates with proteins like Jagged1 and Delta-like ligands which are imperative to the signaling cascade. Cross-talk between these pathways highlights its importance in ensuring precise regulation of cellular functions and maintaining tissue integrity.

Associated diseases and disorders

Notch2 has links to Alagille syndrome and certain malignancies like breast cancer. Mutations or dysregulation in the Notch2 signaling pathway can lead to these conditions. In Alagille syndrome for instance the interplay between Notch2 and Jagged1 mutations alters bile duct development leading to liver and heart problems. Also its role in various cancers suggests that altered Notch2 signaling contributes to uncontrolled cell proliferation and tumor progression.

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1 product image

  • SDS-PAGE - Recombinant Human Notch2 protein (His tag) (ab276341), expandable thumbnail

    SDS-PAGE - Recombinant Human Notch2 protein (His tag) (ab276341)

    SDS-PAGE analysis of ab276341

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Product protocols

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