Recombinant Human NOTCH3 protein
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Recombinant Human NOTCH3 protein is a Human Fragment protein, in the 47 to 156 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
View Alternative Names
Neurogenic locus notch homolog protein 3, Notch 3, NOTCH3
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human NOTCH3 protein (AB114234)
12.5% SDS-PAGE showing ab114234 at approximately 37.73kDa stained with Coomassie Blue.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NOTCH3 influences various cellular processes including proliferation apoptosis and differentiation. NOTCH3 does not function alone it is part of a regulatory complex that mediates cellular context and fate decisions. The receptor is cleaved upon ligand binding to facilitate intracellular signaling. This signaling is critical in the development and maintenance of vascular integrity which highlights the protein's importance in both physiological and pathological contexts.
Pathways
NOTCH3 plays a role in the NOTCH signaling pathway essential for cell fate determination and the smooth muscle cell differentiation pathway. Ligands such as Jagged and Delta-like proteins trigger NOTCH3 activation. Upon activation the NOTCH3 intracellular domain translocates to the nucleus where it interacts with transcriptional regulators like RBPJ. This interaction modulates the expression of target genes influencing cell differentiation and proliferation.
Specifications
Form
Liquid
General info
Function
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination (PubMed : 15350543). Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).
Sequence similarities
Belongs to the NOTCH family.
Post-translational modifications
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.. Phosphorylated.. Hydroxylated by HIF1AN.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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