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AB117065

Recombinant Human NOXA2/p67phox protein (GST tag N-Terminus)

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Recombinant Human NOXA2/p67phox protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 526 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

NOXA2, P67PHOX, NCF2, Neutrophil cytosol factor 2, NCF-2, 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, Neutrophil NADPH oxidase factor 2, p67-phox

1 Images
SDS-PAGE - Recombinant Human NOXA2/p67phox protein (GST tag N-Terminus) (AB117065)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NOXA2/p67phox protein (GST tag N-Terminus) (AB117065)

12.5% SDS-PAGE showing ab117065 at approximately 83.86 kDa.
Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

P19878

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB109523

Anti-NOXA2/p67phox antibody [EPR5065]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-NOXA2/p67phox antibody [EPR5065] (AB109523)

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Proteins & Peptides

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Recombinant Human Ephrin A3 protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human Ephrin A3 protein (denatured) (His tag N-Terminus) (AB156351)

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Proteins & Peptides

AB185383

Recombinant Mouse Cathepsin E protein

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Proteins & Peptides

AB169913

Recombinant Human RNPS1 protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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Sequence info

[{"sequence":"MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTILKNMTEAEKAFTRSINRDKHLAVAYFQRGMLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFACEVLYNIAFMYAKKEEWKKAEEQLALATSMKSEPRHSKIDKAMECVWKQKLYEPVVIPVGRLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEQTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV","proteinLength":"Full Length","predictedMolecularWeight":"83.86 kDa","actualMolecularWeight":null,"aminoAcidEnd":526,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P19878","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOXA2 also known as p67phox is a subunit of the NADPH oxidase complex essential in producing reactive oxygen species (ROS). The protein weighs about 67 kilodaltons and is expressed in phagocytic cells like neutrophils and macrophages. NOXA2/p67phox interacts closely with other subunits of the NADPH oxidase complex playing a critical role in oxidative burst which is essential in the immune response against pathogens.
Biological function summary

NOXA2 contributes to superoxide production by facilitating electron transfer in the NADPH oxidase complex. This complex is integral to the immune system's ability to target and destroy infectious agents. NOXA2/p67phox forms a complex with other components such as gp91phox and p22phox which are required for full activation of the oxidase. The coordinated action of these complexes ensures effective ROS production which is a vital component of the defense mechanism of immune cells.

Pathways

NOXA2 participates in the respiratory burst pathway an important element of innate immunity. This pathway involves the conversion of oxygen to superoxide a process requiring precise interaction with cytochrome b558 and the Rac protein. NOXA2 is also related to signal transduction pathways including those modulated by protein kinase C which further regulates its activation during the immune response. The orchestration of these pathways allows for an effective immune defense mechanism.

NOXA2 is implicated in chronic granulomatous disease (CGD) where mutations in the components of the NADPH oxidase complex impair ROS production. This leads to frequent infections due to deficient pathogen clearance. NOXA2 is also linked to inflammatory disorders due to its role in excessive ROS production. In that context it connects with other proteins such as p47phox which are also involved in both normal and aberrant immune responses.
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Specifications

Form

Liquid

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General info

Function

Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed : 12207919, PubMed : 38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed : 38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed : 38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (By similarity).

Sequence similarities

Belongs to the NCF2/NOXA1 family.

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Product protocols

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Target data

Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed : 12207919, PubMed : 38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed : 38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed : 38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (By similarity).
See full target information NCF2
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