JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB106873

Recombinant Human NPL protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human NPL protein is a Human Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C1orf13, NPL, N-acetylneuraminate lyase, NALase, N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, Sialate lyase, Sialate-pyruvate lyase, Sialic acid aldolase, Sialic acid lyase

1 Images
SDS-PAGE - Recombinant Human NPL protein (AB106873)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NPL protein (AB106873)

15% SDS-PAGE analysis of 3μg ab106873.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9BXD5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAFPKKKLQGLVAATITPMTENGEINFSVIGQYVDYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFFLKPWTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGATGAVGSTYNYLGKKTNQMLEAFEQKDFSLALNYQFCIQRFINFVVKLGFGVSQTKAIMTLVSGIPMGPPRLPLQKASREFTDSAEAKLKSLDFLSFTDLKDGNLEAGS","proteinLength":"Full Length","predictedMolecularWeight":"37.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":320,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BXD5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NPL protein also known as N-acetylneuraminate pyruvate lyase is an enzyme that catalyzes the reversible cleavage of N-acetylneuraminic acid (Neu5Ac) into pyruvate and N-acetylmannosamine. This protein weighs approximately 34 kDa and takes part in the sialic acid metabolism pathway. NPL is mainly expressed in the liver and kidney tissues in humans. It demonstrates a structural requirement for metal ions such as magnesium or manganese as co-factors to stabilize its active form.
Biological function summary

N-acetylneuraminate pyruvate lyase influences the recycling and regulation of sialic acids which are vital components of cell surface molecules involved in cellular recognition and signaling. This protein acts as part of a larger metabolic network involving sialic acid biosynthesis and degradation. It supports cellular functions by recycling sialic acid which gets added to glycoproteins and glycolipids contributing to cell surface architecture and function.

Pathways

The sialic acid metabolism involving the NPL protein connects to the broader glycosylation pathways impacting cell-cell communication and pathogen recognition. The NPL protein interacts with enzymes like sialyltransferases which transfer sialic acids to glycan chains and it maintains the rate of Neu5Ac production and consumption. It also relates to neuraminidases which cleave sialic acids from glycoconjugates ensuring dynamic metabolic fluxes in cells.

The function of NPL protein ties to the regulation of sialic acid levels impacting conditions such as sialuria and macrophage activity in sepsis. Sialuria is associated with excessive Neu5Ac accumulation due to malfunctioning NPL. Additionally altered sialic acid levels can affect immune responses in sepsis where NPL indirectly influences pathways involving other proteins such as sialoadhesins which bind to sialic acids on cell surfaces.
style
left-column

Specifications

Form

Liquid

Additional notes

ab106873 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate (PubMed : 33895133). It prevents sialic acids from being recycled and returning to the cell surface (PubMed : 33895133). Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway (PubMed : 22692205, PubMed : 33895133). Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (Probable).

Sequence similarities

Belongs to the DapA family. NanA subfamily.

style
left-column

Product protocols

style
left-column

Target data

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate (PubMed : 33895133). It prevents sialic acids from being recycled and returning to the cell surface (PubMed : 33895133). Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway (PubMed : 22692205, PubMed : 33895133). Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (Probable).
See full target information NPL
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB223521

Anti-NPL antibody

Immunocytochemistry/ Immunofluorescence - Anti-NPL antibody (AB223521)

  • 0
  • 0
Secondary Antibodies

AB150191

Rabbit Anti-Human IgM mu chain (Alexa Fluor® 647)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Human IgM mu chain (Alexa Fluor® 647) (AB150191)

  • 0
  • 0
Secondary Antibodies

AB99811

Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP)

VeriBlot

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com