Recombinant Human NPL4 protein is a Human Full Length protein, in the 1 to 608 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A E S I I I R V Q S P D G V K R I T A T K R E T A A T F L K K V A K E F G F Q N N G F S V Y I N R N K T G E I T A S S N K S L N L L K I K H G D L L F L F P S S L A G P S S E M E T S V P P G F K V F G A P N V V E D E I D Q Y L S K Q D G K I Y R S R D P Q L C R H G P L G K C V H C V P L E P F D E D Y L N H L E P P V K H M S F H A Y I R K L T G G A D K G K F V A L E N I S C K I K S G C E G H L P W P N G I C T K C Q P S A I T L N R Q K Y R H V D N I M F E N H T V A D R F L D F W R K T G N Q H F G Y L Y G R Y T E H K D I P L G I R A E V A A I Y E P P Q I G T Q N S L E L L E D P K A E V V D E I A A K L G L R K V G W I F T D L V S E D T R K G T V R Y S R N K D T Y F L S S E E C I T A G D F Q N K H P N M C R L S P D G H F G S K F V T A V A T G G P D N Q V H F E G Y Q V S N Q C M A L V R D E C L L P C K D A P E L G Y A K E S S S E Q Y V P D V F Y K D V D K F G N E I T Q L A R P L P V E Y L I I D I T T T F P K D P V Y T F S I S Q N P F P I E N R D V L G E T Q D F H S L A T Y L S Q N T S S V F L D T I S D F H L L L F L V T N E V M P L Q D S I S L L L E A V R T R N E E L A Q T W K R S E Q W A T I E Q L C S T V G G Q L P G L H E Y G A V G G S T H T A T A A M W A C Q H C T F M N Q P G T G H C E M C S L P R T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI (PubMed:26471729).
Alternative names
KIAA1499, NPL4, NPLOC4, Nuclear protein localization protein 4 homolog, Protein NPL4
Recommended products
Recombinant Human NPL4 protein is a Human Full Length protein, in the 1 to 608 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q8TAT6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A E S I I I R V Q S P D G V K R I T A T K R E T A A T F L K K V A K E F G F Q N N G F S V Y I N R N K T G E I T A S S N K S L N L L K I K H G D L L F L F P S S L A G P S S E M E T S V P P G F K V F G A P N V V E D E I D Q Y L S K Q D G K I Y R S R D P Q L C R H G P L G K C V H C V P L E P F D E D Y L N H L E P P V K H M S F H A Y I R K L T G G A D K G K F V A L E N I S C K I K S G C E G H L P W P N G I C T K C Q P S A I T L N R Q K Y R H V D N I M F E N H T V A D R F L D F W R K T G N Q H F G Y L Y G R Y T E H K D I P L G I R A E V A A I Y E P P Q I G T Q N S L E L L E D P K A E V V D E I A A K L G L R K V G W I F T D L V S E D T R K G T V R Y S R N K D T Y F L S S E E C I T A G D F Q N K H P N M C R L S P D G H F G S K F V T A V A T G G P D N Q V H F E G Y Q V S N Q C M A L V R D E C L L P C K D A P E L G Y A K E S S S E Q Y V P D V F Y K D V D K F G N E I T Q L A R P L P V E Y L I I D I T T T F P K D P V Y T F S I S Q N P F P I E N R D V L G E T Q D F H S L A T Y L S Q N T S S V F L D T I S D F H L L L F L V T N E V M P L Q D S I S L L L E A V R T R N E E L A Q T W K R S E Q W A T I E Q L C S T V G G Q L P G L H E Y G A V G G S T H T A T A A M W A C Q H C T F M N Q P G T G H C E M C S L P R T
- Accession
- Q8TAT6
- Protein length
- Full Length
- Amino acids
- 1 to 608
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI (PubMed:26471729).
- Sequence similarities
Belongs to the NPL4 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein NPL4 known also as Nuclear Protein Localization 4 is involved in key cellular processes as a cofactor in the p97 ATPase complex. It has a molecular mass of approximately 68 kDa. NPL4 is expressed in different tissues with increased levels observed in dividing cells. Mechanically NPL4 aids in protein degradation by advancing the extraction of ubiquitinated substrates from the ER-associated degradation (ERAD) pathway.
- Biological function summary
NPL4 participates in the unfoldase activity required for the ERAD process. It functions within a complex that includes p97/VCP and other cofactors like UFD1. This complex targets misfolded proteins for degradation in the proteasome maintaining cellular proteostasis. In a broader scope NPL4 ensures efficient cellular response to protein stress which is necessary for normal cell cycle progression and mitosis.
- Pathways
NPL4 integrates into essential proteostasis pathways interplaying with ERAD and the ubiquitin-proteasome system. It interacts with key proteins like UFD1 and p97/VCP within these pathways. The NPL4-p97-VCP complex facilitates protein dislocation during ER stress therefore influencing the unfolded protein response (UPR) a critical cellular adaptation mechanism.
- Associated diseases and disorders
Malfunction of NPL4 has links to neurodegenerative diseases and certain cancers. Impairments in NPL4 function and its complex with p97/VCP associate with the accumulation of ubiquitinated proteins which contributes to pathological states such as amyotrophic lateral sclerosis (ALS) and various tumor progressions. Mutations within proteins of this complex also play a role in those conditions underpinning the critical importance of NPL4 in cellular health.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human NPL4 protein (ab153405)
ab153405 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com