Recombinant Human NQO2 protein is a Human Full Length protein, in the 1 to 231 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Publications
- Communications biology 5:1572022Fortilin interacts with TGF-β1 and prevents TGF-β receptor activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDecha Pinkaew,Erik Martinez-Hackert,Wei Jia,Matthew D King,Fei Miao,Nicole R Enger,Runglawan Silakit,Kota Ramana,Shi-You Chen,Ken FujisePubMed 35197550
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H < / b > M A G K K V L I V Y A H Q E P K S F N G S L K N V A V D E L S R Q G C T V T V S D L Y A M N F E P R A T D K D I T G T L S N P E V F N Y G V E T H E A Y K Q R S L A S D I T D E Q K K V R E A D L V I F Q F P L Y W F S V P A I L K G W M D R V L C Q G F A F D I P G F Y D S G L L Q G K L A L L S V T T G G T A E M Y T K T G V N G D S R Y F L W P L Q H G T L H F C G F K V L A P Q I S F A P E I A S E E E R K G M V A A W S Q R L Q T I W K E E P I P C T A H W H F G Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Alternative names
Ribosyldihydronicotinamide dehydrogenase [quinone], NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2, NMOR2, NQO2
Recommended products
Recombinant Human NQO2 protein is a Human Full Length protein, in the 1 to 231 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Alternative names
Ribosyldihydronicotinamide dehydrogenase [quinone], NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2, NMOR2, NQO2
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- P16083-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H < / b > M A G K K V L I V Y A H Q E P K S F N G S L K N V A V D E L S R Q G C T V T V S D L Y A M N F E P R A T D K D I T G T L S N P E V F N Y G V E T H E A Y K Q R S L A S D I T D E Q K K V R E A D L V I F Q F P L Y W F S V P A I L K G W M D R V L C Q G F A F D I P G F Y D S G L L Q G K L A L L S V T T G G T A E M Y T K T G V N G D S R Y F L W P L Q H G T L H F C G F K V L A P Q I S F A P E I A S E E E R K G M V A A W S Q R L Q T I W K E E P I P C T A H W H F G Q
- Accession
- Protein length
Full Length
- Predicted molecular weight
28.1 kDa
- Amino acids
1 to 231
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab93933 was purified using conventional chromatography techniques.
General info
- Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
- Sequence similarities
Belongs to the NAD(P)H dehydrogenase (quinone) family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
- Activity summary
NQO2 also known as NRH:quinone oxidoreductase 2 is a flavoprotein enzyme playing roles in cellular redox reactions. It has a molecular mass of approximately 25 kDa. NQO2 is expressed widely in human tissues such as liver colon and brain. It catalyzes the two-electron reduction of quinones to hydroquinones therefore helping prevent oxidative stress by converting reactive quinone species into less reactive and more stable molecules. The protein often interacts with other cellular components to perform its functions effectively.
- Biological function summary
NQO2 participates in detoxification processes and acts as a dihydronicotinamide riboside:quinone oxidoreductase. Although it is not part of a large protein complex NQO2 may associate with other molecules or enzymes that modulate cellular antioxidant defense systems. It serves as a backup system when other primary antioxidant enzymes such as NQO1 are impaired. This function reflects the importance of NQO2 in maintaining cellular health and response to environmental stresses.
- Pathways
NQO2 is involved in metabolism and oxidative stress response pathways. Its role in quinone detoxification connects it to the cellular defense mechanism against oxidative damage interacting with proteins like NQO1. NQO2's enzymatic activity goes hand-in-hand with the NAD(P)H dehydrogenase (quinone) pathway which collaborates with other proteins to maintain redox homeostasis and protect cells against electrophilic and oxidative stress.
- Associated diseases and disorders
Alterations in NQO2 expression or function are linked to cancer and neurodegenerative diseases. NQO2's involvement in detoxifying potent quinones links it to cancerous developments when these processes fail while its role against oxidative stress connects it to neurodegenerative conditions like Parkinson’s disease. During these conditions interactions with proteins such as NQO1 and p53 can be significant. The balance of NQO2 activity may contribute to disease progression or suppression highlighting its therapeutic potential in disease management.
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SDS-PAGE - Recombinant Human NQO2 protein (ab93933)
15% SDS-PAGE analysis of 3μg ab93933
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