Recombinant Human NR0B1 / Dax1 protein is a Human Full Length protein, in the 1 to 470 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A G E N H Q W Q G S I L Y N M L M S A K Q T R A A P E A P E T R L V D Q C W G C S C G D E P G V G R E G L L G G R N V A L L Y R C C F C G K D H P R Q G S I L Y S M L T S A K Q T Y A A P K A P E A T L G P C W G C S C G S D P G V G R A G L P G G R P V A L L Y R C C F C G E D H P R Q G S I L Y S L L T S S K Q T H V A P A A P E A R P G G A W W D R S Y F A Q R P G G K E A L P G G R A T A L L Y R C C F C G E D H P Q Q G S T L Y C V P T S T N Q A Q A A P E E R P R A P W W D T S S G A L R P V A L K S P Q V V C E A A S A G L L K T L R F V K Y L P C F Q V L P L D Q Q L V L V R N C W A S L L M L E L A Q D R L Q F E T V E V S E P S M L Q K I L T T R R R E T G G N E P L P V P T L Q H H L A P P A E A R K V P S A S Q V Q A I K C F L S K C W S L N I S T K E Y A Y L K G T V L F N P D V P G L Q C V K Y I Q G L Q W G T Q Q I L S E H T R M T H Q G P H D R F I E L N S T L F L L R F I N A N V I A E L F F R P I I G T V S M D D M M L E M L C T K I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).
Alternative names
AHC, DAX1, NR0B1, Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1
Recommended products
Recombinant Human NR0B1 / Dax1 protein is a Human Full Length protein, in the 1 to 470 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P51843-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A G E N H Q W Q G S I L Y N M L M S A K Q T R A A P E A P E T R L V D Q C W G C S C G D E P G V G R E G L L G G R N V A L L Y R C C F C G K D H P R Q G S I L Y S M L T S A K Q T Y A A P K A P E A T L G P C W G C S C G S D P G V G R A G L P G G R P V A L L Y R C C F C G E D H P R Q G S I L Y S L L T S S K Q T H V A P A A P E A R P G G A W W D R S Y F A Q R P G G K E A L P G G R A T A L L Y R C C F C G E D H P Q Q G S T L Y C V P T S T N Q A Q A A P E E R P R A P W W D T S S G A L R P V A L K S P Q V V C E A A S A G L L K T L R F V K Y L P C F Q V L P L D Q Q L V L V R N C W A S L L M L E L A Q D R L Q F E T V E V S E P S M L Q K I L T T R R R E T G G N E P L P V P T L Q H H L A P P A E A R K V P S A S Q V Q A I K C F L S K C W S L N I S T K E Y A Y L K G T V L F N P D V P G L Q C V K Y I Q G L Q W G T Q Q I L S E H T R M T H Q G P H D R F I E L N S T L F L L R F I N A N V I A E L F F R P I I G T V S M D D M M L E M L C T K I
- Accession
- P51843
- Protein length
- Full Length
- Predicted molecular weight
- 78.1 kDa
- Amino acids
- 1 to 470
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Glutathione Sepharose 4 Fast Flow
General info
- Function
Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).
- Sequence similarities
Belongs to the nuclear hormone receptor family. NR0 subfamily.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NR0B1 also known as Dax1 is a nuclear receptor protein that acts as a transcriptional regulator. It has an approximate mass of 51 kDa and plays an important role in modulating gene expression. This protein is highly expressed in tissues such as the adrenal gland hypothalamus testis and ovary where it exerts its regulatory functions. Although NR0B1 lacks a classical DNA-binding domain it interacts with other proteins to conduct its mechanical functions.
- Biological function summary
NR0B1 interacts with other nuclear receptors and transcription factors to regulate steroidogenesis and reproductive development. It plays a critical role in maintaining the balance of transcriptional activity within these systems. NR0B1 functions as a corepressor and participates in protein complexes modulating the activity of specific gene targets. Its biological activities ensure proper development and differentiation of sex-specific organs.
- Pathways
NR0B1 is actively involved in the steroid hormone biosynthesis and Wnt signaling pathways. In these pathways NR0B1 influences various downstream processes by interacting with proteins such as SF1 and Wnt components. NR0B1 helps coordinate the activities of other transcriptional regulators effectively integrating signals within the pathway to control cellular outcomes.
- Associated diseases and disorders
NR0B1 mutations or dysfunctions can cause conditions such as congenital adrenal hypoplasia and hypogonadotropic hypogonadism. Altered NR0B1 activity interferes with normal adrenal gland and reproductive system development. Its interaction with proteins like SF1 further connects it to pathologies in these systems. Understanding NR0B1's role in these diseases can help develop targeted therapies to address the underlying genetic or functional disruptions.
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1 product image
SDS-PAGE - Recombinant Human NR0B1 / Dax1 protein (ab152174)
12.5% SDS-PAGE analysis of ab152174 stained with Coomassie Blue.
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