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AB132488

Recombinant Human NR0B2 protein

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Recombinant Human NR0B2 protein is a Human Full Length protein, in the 1 to 257 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

SHP, NR0B2, Nuclear receptor subfamily 0 group B member 2, Orphan nuclear receptor SHP, Small heterodimer partner

1 Images
SDS-PAGE - Recombinant Human NR0B2 protein (AB132488)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NR0B2 protein (AB132488)

12.5% SDS-PAGE analysis of ab132488 stained with Coomassie Blue

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

Q15466

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSTSQPGACPCQGAASRPAILYALLSSSLKAVPRPRSRCLCRQHRPVQLCAPHRTCREALDVLAKTVAFLRNLPSFWQLPPQDQRRLLQGCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEPSSSGGSGQLPDRPQPSLAAVQWLQCCLESFWSLELSPKEYACLKGTILFNPDVPGLQAASHIGHLQQEAHWVLCEVLEPWCPAAQGRLTRVLLTASTLKSIPTSLLGDLFFRPIIGDVDIAGLLGDMLLLR","proteinLength":"Full Length","predictedMolecularWeight":"54.01 kDa","actualMolecularWeight":null,"aminoAcidEnd":257,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q15466","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NR0B2 also known as small heterodimer partner (SHP) is a nuclear receptor that functions as a transcriptional regulator. It lacks a conventional DNA-binding domain distinguishing it mechanistically from other nuclear receptors. SHP weighs approximately 28 kDa. It is broadly expressed within the liver pancreas spleen and adrenal gland. In these tissues NR0B2 acts by interacting with other transcription factors to repress the transcription of specific target genes influencing various metabolic processes.
Biological function summary

NR0B2 modulates diverse processes such as bile acid synthesis glucose homeostasis and lipid metabolism. It operates alone and does not form part of a complex. Instead it directly interacts with other nuclear receptors and transcription factors including liver receptor homolog-1 (LRH-1) and retinoid X receptor (RXR) to control gene expression linked to metabolism. Through these interactions SHP acts as a vital checkpoint to maintain metabolic stability within the body.

Pathways

NR0B2 is an important regulator in bile acid and gluconeogenesis pathways. Through its interaction with LRH-1 NR0B2 negatively regulates the bile acid synthesis pathway reducing bile acid production when levels are already sufficient. In terms of gluconeogenesis NR0B2 works alongside forkhead box protein O1 (FoxO1) to manage glucose levels in the liver. This control is critical for energy homeostasis responding to the body's nutritional states.

NR0B2 involvement links to metabolic syndromes and non-alcoholic fatty liver disease (NAFLD). Higher or lower levels of NR0B2 influence the development of these conditions by affecting cholesterol and triglyceride storage. SHP's interactions with proteins like peroxisome proliferator-activated receptor gamma (PPARγ) are significant as these can further disrupt lipid metabolism and enhance the risk or severity of these diseases. Understanding NR0B2's role offers pathways for therapeutic interventions focusing on these metabolic disorders.
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Specifications

Form

Liquid

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General info

Function

Transcriptional regulator that acts as a negative regulator of receptor-dependent signaling pathways (By similarity). Specifically inhibits transactivation of the nuclear receptor with which it interacts (By similarity). Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1 (PubMed : 14752053). Essential component of the liver circadian clock which via its interaction with NR1D1 and RORG regulates NPAS2-mediated hepatic lipid metabolism (By similarity). Regulates the circadian expression of cytochrome P450 (CYP) enzymes (By similarity). Represses : NR5A2 and HNF4A to down-regulate CYP2C38, NFLI3 to up-regulate CYP2A5, BHLHE41/HNF1A axis to up-regulate CYP1A2, CYP2E1 and CYP3A11, and NR1D1 to up-regulate CYP2B10, CYP4A10 and CYP4A14 (By similarity).

Sequence similarities

Belongs to the nuclear hormone receptor family. NR0 subfamily.

Post-translational modifications

Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcriptional regulator that acts as a negative regulator of receptor-dependent signaling pathways (By similarity). Specifically inhibits transactivation of the nuclear receptor with which it interacts (By similarity). Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1 (PubMed : 14752053). Essential component of the liver circadian clock which via its interaction with NR1D1 and RORG regulates NPAS2-mediated hepatic lipid metabolism (By similarity). Regulates the circadian expression of cytochrome P450 (CYP) enzymes (By similarity). Represses : NR5A2 and HNF4A to down-regulate CYP2C38, NFLI3 to up-regulate CYP2A5, BHLHE41/HNF1A axis to up-regulate CYP1A2, CYP2E1 and CYP3A11, and NR1D1 to up-regulate CYP2B10, CYP4A10 and CYP4A14 (By similarity).
See full target information NR0B2
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