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AB82070

Recombinant Human NR5A2 / LRH1 protein

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Recombinant Human NR5A2 / LRH1 protein is a Human Full Length protein, expressed in Baculovirus, with >95%, suitable for SDS-PAGE.

View Alternative Names

B1F, CPF, FTF, NR5A2, Nuclear receptor subfamily 5 group A member 2, Alpha-1-fetoprotein transcription factor, B1-binding factor, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, hB1F, LRH-1

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O00482

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.9 Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

1 unit equals 1 nanogram of purified protein.
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Sequence info

[{"sequence":"MSSNSDTGDLQESLKHGLTPIGAGLPDRHGSPIPARGRLVMLPKVETEALGLARSHGEQGQMPENMQVSQFKMVNYSYDEDLEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKRYTCIENQNCQIDKTQRKRCPYCRFQKCLSVGMKLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALIRANGLKLEAMSQVIQAMPSDLTISSAIQNIHSASKGLPLNHAALPPTDYDRSPFVTSPISMTMPPHGSLQGYQTYGHFPSRAIKSEYPDPYTSSPESIMGYSYMDSYQTSSPASIPHLILELLKCEPDEPQVQAKIMAYLQQEQANRSKHEKLSTFGLMCKMADQTLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVVHGKEGSIFLVTGQQVDYSIIASQAGATLNNLMSHAQELVAKLRSLQFDQREFVCLKFLVLFSLDVKNLENFQLVEGVQEQVNAALLDYTMCNYPQQTEKFGQLLLRLPEIRAISMQAEEYLYYKHLNGDVPYNNLLIEMLHAKRA","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O00482","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NR5A2 also known as Liver Receptor Homolog-1 (LRH-1) is a nuclear receptor protein with a molecular weight of approximately 55 kDa. It regulates gene expression by binding to DNA response elements as a monomer. NR5A2 is expressed in the liver pancreas ovary and intestine. This receptor plays a central role in the transcriptional regulation of genes involved in important metabolic processes such as cholesterol homeostasis bile acid synthesis and steroidogenesis.
Biological function summary

NR5A2 influences several physiological functions by modulating the expression of key genes. It is a part of the nuclear receptor superfamily and acts as a transcriptional regulator. This receptor directly impacts the regulation of genes that control metabolism and energy balance. Its role in lipid metabolism has been observed as NR5A2 regulates genes encoding enzymes for cholesterol and bile acid biosynthesis linking it to energy homeostasis.

Pathways

The protein modulates essential biological processes involved in energy and lipid metabolism linking it to the Wnt/β-catenin signaling and cholesterol metabolism pathways. It interacts with signaling proteins such as β-catenin where it is involved in the transcriptional regulation of genes necessary for normal cellular function. NR5A2 through these pathways maintains homeostasis and influences cellular responses to metabolic changes highlighting its interaction within complex signaling networks.

NR5A2 has significant associations with metabolic diseases and cancers like pancreatic cancer. Mutations or dysregulations in NR5A2 expression levels can affect cholesterol metabolism potentially contributing to conditions such as non-alcoholic fatty liver disease (NAFLD). This receptor's expression levels influence the expression of genes that are pivotal in cancer development and its regulatory role may be linked to c-Myc a protein involved in cell growth and proliferation within these diseases.
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Specifications

Form

Liquid

Additional notes

Purified by affinity and FPLC chromatography.

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General info

Function

Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor : inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed : 20159957). May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38 (By similarity).. (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with HNF1A to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).

Sequence similarities

Belongs to the nuclear hormone receptor family. NR5 subfamily.

Post-translational modifications

Sumoylated by SUMO1 at Lys-270 during the hepatic acute phase response, leading to promote interaction with GPS2 and prevent N-Cor corepressor complex dissociation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor : inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed : 20159957). May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38 (By similarity).. (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with HNF1A to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).
See full target information NR5A2
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Complementary Products

Primary Antibodies

AB308286

Anti-NR5A2 / LRH1 antibody [EPR26987-37] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-NR5A2 / LRH1 antibody [EPR26987-37] - BSA and Azide free (Detector) (AB308286)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB99811

Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP)

VeriBlot

  • 5
  • 1

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