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AB171719

Recombinant Human NRBF2 protein (His tag N-Terminus)

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Recombinant Human NRBF2 protein (His tag N-Terminus) is a Human Fragment protein, in the 45 to 287 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

COPR, NRBF2, Nuclear receptor-binding factor 2, NRBF-2, Comodulator of PPAR and RXR

1 Images
SDS-PAGE - Recombinant Human NRBF2 protein (His tag N-Terminus) (AB171719)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NRBF2 protein (His tag N-Terminus) (AB171719)

15% SDS-PAGE analysis of ab171719 (3 μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q96F24

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMKLTQSEQAHLSLELQRDSHMKQLLLIQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYSPSTEKCLPEIQGIFDRDPDTLLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGPIEKELDVDADFVETSELWSLPPHAETATASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMNN","proteinLength":"Fragment","predictedMolecularWeight":"29.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":287,"aminoAcidStart":45,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96F24","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NRBF2 also known as nuclear receptor binding factor 2 acts as an important regulatory protein in cellular mechanisms. It has a molecular mass of approximately 30 kDa and exhibits expression in various tissues such as the brain and liver. Mechanically NRBF2 interacts directly with the Beclin 1 complex facilitating the regulation of autophagy a process critical for cellular homeostasis and survival.
Biological function summary

NRBF2 serves essential roles in controlling autophagic vesicle formation. It forms part of the autophagy-related phosphatidylinositol 3-kinase (PI3K) complex I enhancing the lipid kinase activity required for autophagosome development. This protein-protein interaction is key for initiating the autophagy cascade ensuring that cells can manage and recycle cellular components efficiently under stress conditions.

Pathways

NRBF2 engages in the autophagy pathway where it influences the action of the PI3K complex I. It also partakes in the mTOR signaling pathway where it helps in regulating cellular energy status and protein synthesis. Through these pathways NRBF2 maintains a presence alongside other regulatory proteins like ULK1 and AMPK which mutually contribute to the balance of cell growth and metabolism.

Loss or dysfunction of NRBF2 links closely to neurological diseases and cancer. For instance its impaired function has associations with neurodegenerative disorders such as Alzheimer's disease due to its role in neuronal autophagy and associated proteins like LC3. Additionally the altered activity of NRBF2 within pathways can promote oncogenesis through connections with tumor suppressor proteins making it a point of interest in cancer research.
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Specifications

Form

Liquid

Additional notes

ab171719 was purified by anion-exchange and gel-filtration chromatography techniques.

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General info

Function

May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).. Involved in starvation-induced autophagy probably by its association with PI3K complex I (PI3KC3-C1). However, effects has been described variably. Involved in the induction of starvation-induced autophagy (PubMed : 24785657). Stabilizes PI3KC3-C1 assembly and enhances ATG14-linked lipid kinase activity of PIK3C3 (By similarity). Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1 (PubMed : 25086043). May be involved in autophagosome biogenesis (PubMed : 25086043). May play a role in neural progenitor cell survival during differentiation (By similarity).

Subcellular localisation

Nucleus

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Product protocols

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Target data

May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).. Involved in starvation-induced autophagy probably by its association with PI3K complex I (PI3KC3-C1). However, effects has been described variably. Involved in the induction of starvation-induced autophagy (PubMed : 24785657). Stabilizes PI3KC3-C1 assembly and enhances ATG14-linked lipid kinase activity of PIK3C3 (By similarity). Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1 (PubMed : 25086043). May be involved in autophagosome biogenesis (PubMed : 25086043). May play a role in neural progenitor cell survival during differentiation (By similarity).
See full target information NRBF2
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