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AB132404

Recombinant Human NRF1 protein

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(1 Publication)

Recombinant Human NRF1 protein is a Human Full Length protein, in the 1 to 522 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1

1 Images
SDS-PAGE - Recombinant Human NRF1 protein (AB132404)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NRF1 protein (AB132404)

12.5% SDS-PAGE analysis of ab132404 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

Q16656

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MEEHGVTQTEHMATIEAHAVAQQVQQVHVATYTEHSMLSADEDSPSSPEDTSYDDSDILNSTAADEVTAHLAAAGPVGMAAAAAVATGKKRKRPHVFESNPSIRKRQQTRLLRKLRATLDEYTTRVGQQAIVLCISPSKPNPVFKVFGAAPLENVVRKYKSMILEDLESALAEHAPAPQEVNSELPPLTIDGIPVSVDKMTQAQLRAFIPEMLKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ","proteinLength":"Full Length","predictedMolecularWeight":"82.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":522,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q16656","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The nuclear respiratory factor 1 (NRF1) also known as Alpha-Pal is a transcription factor with an approximate molecular weight of 68 kDa. It plays an important role in the regulation of genes required for mitochondrial DNA transcription and replication. Researchers find NRF1 expression in various tissues including muscle brain and liver. This protein functions mechanistically by binding to specific promoter regions of target genes to control their expression making it essential for maintaining cellular energy metabolism.
Biological function summary

NRF1 influences the expression of genes involved in the mitochondrial function and is part of the transcriptional complex. This complex includes the NRF2 protein where NRF1 partners with co-activators to modulate gene transcription. NRF1 promotes the expression of components essential for mitochondrial biogenesis and cellular respiration which are vital processes for energy production in cells. Therefore NRF1 contributes to maintaining cellular energy homeostasis and overall cellular health.

Pathways

NRF1 integrates into critical regulatory pathways for mitochondrial function and metabolism. NRF1 links closely to the PGC-1α pathway which is instrumental in the regulation of energy metabolism and mitochondrial biogenesis. Additionally NRF1 works in conjunction with NRF2 to regulate oxidative stress responses. These interactions ensure a coordinated cellular response to energy demands and help protect the cell from oxidative damage.

NRF1 associates with neurodegenerative diseases and metabolic syndromes. Altered NRF1 regulation can impact diseases such as Alzheimer's disease where it may contribute to neurodegeneration through impaired mitochondrial function. Moreover the dysfunction of NRF1 might relate to metabolic disorders like diabetes as energy metabolism and mitochondrial health are disrupted. NRF1 connects with proteins like SIRT1 in these contexts further implicating NRF1 in these pathological states through its role in energy homeostasis and stress response.
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Specifications

Form

Liquid

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General info

Function

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Sequence similarities

Belongs to the NRF1/Ewg family.

Post-translational modifications

Phosphorylation enhances DNA binding.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
See full target information NRF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:3337 PubMed34099689

2021

High throughput screening identifies SOX2 as a super pioneer factor that inhibits DNA methylation maintenance at its binding sites.

Applications

Unspecified application

Species

Unspecified reactive species

Ludovica Vanzan,Hadrien Soldati,Victor Ythier,Santosh Anand,Simon M G Braun,Nicole Francis,Rabih Murr
View all publications
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Complementary Products

Primary Antibodies

AB175932

Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (AB175932)

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Primary Antibodies

AB72230

Anti-PGC1 alpha + beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (AB72230)

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  • 2
Primary Antibodies

AB176558

Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker

RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (AB176558)

  • 5
  • 1
Primary Antibodies

AB124773

Anti-Mitofusin 2 antibody [NIAR164]

KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (AB124773)

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Product promise

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