Recombinant human NRG1 protein (Active)
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Recombinant human NRG1 protein (Active) is a Human Fragment protein, in the 20 to 242 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
View Alternative Names
GGF, HGL, HRGA, NDF, SMDF, NRG1, Pro-NRG1
- FuncS
Supplier Data
Functional Studies - Recombinant human NRG1 protein (Active) (AB282401)
Fully biologically active determined by the dose dependent proliferation of MCF-7 cells.
ED50 is ≤0.15 ng/mL corresponding to a specific actvity of 6.66 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3394381-1
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human NRG1 protein (Active) (AB282401)
SDS-page analysis of ab282401
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NRG1 is involved in several cellular activities such as cell growth survival and differentiation. It is not part of a complex but acts directly through its interactions with receptor tyrosine kinases in the ErbB receptor family. NRG1 influences cardiac development and neural function by mediating these cellular processes promoting the development and maintenance of neural and cardiac tissues.
Pathways
NRG1 is involved in the PI3K-Akt and MAPK/ERK signaling pathways. These pathways are critical for cellular growth differentiation and survival supporting the functions of NRG1 in various tissues. The interaction with ErbB2 and ErbB3 receptors is especially significant for these pathways as they help regulate the cellular responses initiated by NRG1 signaling.
Specifications
Form
Lyophilized
General info
Function
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4 (PubMed : 10867024, PubMed : 7902537). Binds to ERBB3 (PubMed : 20682778). Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV : ITGB3 or ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 20682778). Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (By similarity).
Sequence similarities
Belongs to the neuregulin family.
Post-translational modifications
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage (By similarity).
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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