Recombinant Human NRG1 type II protein (Fc Chimera) is a Human Fragment protein, in the 2 to 246 aa range, expressed in HEK 293, with >88% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >88% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S E R K E G R G K G K G K K K E R G S G K K P E S A A G S Q S P A L P P R L K E M K S Q E S A A G S K L V L R C E T S S E Y S S L R F K W F K N G N E L N R K N K P Q N I K I Q K K P G K S E L R I N K A S L A D S G E Y M C K V I S K L G N D S A S A N I T I V E S N E I I T G M P A S T E G A Y V S S E S P I R I S V S T E G A N T S S S T S T S T T G T S H L V K C A E K E K T F C V N G G E C F M V K D L S N P S R Y L C K C Q P G F T G A R C T E N V P M K V Q N Q E K A E E L Y Q K R V L T I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4 (PubMed:10867024, PubMed:7902537). Binds to ERBB3 (PubMed:20682778). Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:20682778). Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (By similarity).
Alternative names
GGF, HGL, HRGA, NDF, SMDF, NRG1, Pro-NRG1
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Recombinant Human NRG1 type II protein (Fc Chimera) is a Human Fragment protein, in the 2 to 246 aa range, expressed in HEK 293, with >88% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >88% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q02297-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- S E R K E G R G K G K G K K K E R G S G K K P E S A A G S Q S P A L P P R L K E M K S Q E S A A G S K L V L R C E T S S E Y S S L R F K W F K N G N E L N R K N K P Q N I K I Q K K P G K S E L R I N K A S L A D S G E Y M C K V I S K L G N D S A S A N I T I V E S N E I I T G M P A S T E G A Y V S S E S P I R I S V S T E G A N T S S S T S T S T T G T S H L V K C A E K E K T F C V N G G E C F M V K D L S N P S R Y L C K C Q P G F T G A R C T E N V P M K V Q N Q E K A E E L Y Q K R V L T I
- Accession
- Q02297
- Protein length
- Fragment
- Predicted molecular weight
- 53.4 kDa
- Amino acids
- 2 to 246
- Nature
- Recombinant
- Tags
- Fc tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4 (PubMed:10867024, PubMed:7902537). Binds to ERBB3 (PubMed:20682778). Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:20682778). Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (By similarity).
- Sequence similarities
Belongs to the neuregulin family.
- Post-translational modifications
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NRG1 type II also known as Neuregulin-1 type II or heregulin is part of the neuregulin family of proteins. It plays an important role in cell signaling by acting as a ligand for ErbB family receptors which are tyrosine kinase receptors. The protein has a mass of approximately 44 kDa. NRG1 type II is expressed in a variety of tissues such as the nervous system heart and skeletal muscles where it helps in cellular communication.
- Biological function summary
This protein is essential for processes like cell growth differentiation and survival. NRG1 type II is often involved in the formation of multiprotein signaling complexes specifically with ErbB3 and ErbB4 receptors. This interaction can initiate cellular responses critical for the proper functioning of the nervous system and cardiac tissue development. These functions underline the importance of NRG1 type II in maintaining the normal physiological state of these tissues.
- Pathways
You find NRG1 type II involved in significant signaling pathways such as the PI3K/AKT and MAPK/ERK pathways. These pathways are essential for regulating cell proliferation and survival. In these signaling cascades NRG1 type II interacts primarily with the ErbB receptor family including proteins such as ErbB2 and ErbB4 forming heterodimers that activate downstream signaling processes essential for cellular health and function.
- Associated diseases and disorders
NRG1 type II has associations with conditions like schizophrenia and breast cancer. In schizophrenia altered NRG1 type II signaling has been implicated in disrupting neuronal connectivity and synaptic functionality often related to changes in ErbB4 interactions. Regarding breast cancer abnormal expression or mutation of NRG1 type II can lead to excessive activation of the ErbB2 receptor which can promote tumorigenic processes. Understanding these connections is valuable for developing targeted therapeutics for these diseases.
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SDS-PAGE - Recombinant Human NRG1 type II protein (Fc Chimera) (ab276410)
SDS-PAGE analysis of ab276410
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