Recombinant Human NRK1 protein (His tag N-Terminus)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NRK1 also known as nicotinamide riboside kinase 1 is an enzyme involved in the NAD+ biosynthesis pathway. It functions by phosphorylating nicotinamide riboside (NR) to generate nicotinamide mononucleotide (NMN) an important precursor for NAD+ synthesis. The molecular mass of NRK1 is approximately 34 kDa. This protein is expressed in various tissues including liver brain and muscle where it contributes to the regulation of cellular NAD+ levels.

Biological function summary

This protein plays an important role in cellular energy metabolism and regulation. NRK1 is not part of a larger complex but interacts directly with its substrate nicotinamide riboside. By catalyzing the conversion of NR to NMN NRK1 supports the maintenance of NAD+ levels inside cells which is essential for numerous metabolic processes including those related to energy production and cellular stress responses. NRK1 activity helps regulate critical cellular functions such as DNA repair and mitochondrial function.

Pathways

Nicotinamide riboside kinase 1 integrates into the NAD+ biosynthesis pathway. This pathway is important for maintaining adequate NAD+ levels necessary for various enzymes like sirtuins and poly(ADP-ribose) polymerases (PARPs) that rely on NAD+ as a co-factor. The NRK1-mediated conversion process is closely related to other key proteins such as NRK2 which shares similar enzymatic activity and roles in NAD+ metabolism. Together these proteins contribute to the cellular mechanisms that control energy metabolism and stress responses.

Nicotinamide riboside kinase 1 has shown relevance to metabolic disorders such as obesity and diabetes. Dysregulation of NAD+ metabolism partly attributed to altered NRK1 activity can influence the onset and progression of these conditions. Additionally the role of NRK1 in supporting healthy NAD+ levels connects it to proteins like SIRT1 which mediates beneficial effects on metabolism and longevity. Understanding NRK1's involvement in these pathways may advance therapeutic strategies for metabolic and age-related diseases.