Recombinant human NRP2 protein (Fc Chimera) is a Human Fragment protein, in the 1 to 855 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M D M F P L T W V F L A L Y F S R H Q V R G Q P D P P C G G R L N S K D A G Y I T S P G Y P Q D Y P S H Q N C E W I V Y A P E P N Q K I V L N F N P H F E I E K H D C K Y D F I E I R D G D S E S A D L L G K H C G N I A P P T I I S S G S M L Y I K F T S D Y A R Q G A G F S L R Y E I F K T G S E D C S K N F T S P N G T I E S P G F P E K Y P H N L D C T F T I L A K P K M E I I L Q F L I F D L E H D P L Q V G E G D C K Y D W L D I W D G I P H V G P L I G K Y C G T K T P S E L R S S T G I L S L T F H T D M A V A K D G F S A R Y Y L V H Q E P L E N F Q C N V P L G M E S G R I A N E Q I S A S S T Y S D G R W T P Q Q S R L H G D D N G W T P N L D S N K E Y L Q V D L R F L T M L T A I A T Q G A I S R E T Q N G Y Y V K S Y K L E V S T N G E D W M V Y R H G K N H K V F Q A N N D A T E V V L N K L H A P L L T R F V R I R P Q T W H S G I A L R L E L F G C R V T D A P C S N M L G M L S G L I A D S Q I S A S S T Q E Y L W S P S A A R L V S S R S G W F P R I P Q A Q P G E E W L Q V D L G T P K T V K G V I I Q G A R G G D S I T A V E A R A F V R K F K V S Y S L N G K D W E Y I Q D P R T Q Q P K L F E G N M H Y D T P D I R R F D P I P A Q Y V R V Y P E R W S P A G I G M R L E V L G C D W T D S K P T V E T L G P T V K S E E T T T P Y P T E E E A T E C G E N C S F E D D K D L Q L P S G F N C N F D F L E E P C G W M Y D H A K W L R T T W A S S S S P N D R T F P D D R N F L R L Q S D S Q R E G Q Y A R L I S P P V H L P R S P V C M E F Q Y Q A T G G R G V A L Q V V R E A S Q E S K L L W V I R E D Q G G E W K H G R I I L P S Y D M E Y Q I V F E G V I G K G R S G E I A I D D I R I S T D V P L E N C M E P I S A F A V D I P E I H E R E G Y E D E I D D E Y E V D W S N S S S A T S G S G A P S T D K E K S W L Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF. (Microbial infection) Acts as a receptor for human cytomegalovirus pentamer-dependent entry in epithelial and endothelial cells.
Alternative names
VEGF165R2, NRP2, Neuropilin-2, Vascular endothelial cell growth factor 165 receptor 2
Recommended products
Recombinant human NRP2 protein (Fc Chimera) is a Human Fragment protein, in the 1 to 855 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized human VEGFC-His at 10 μg/mL (100 μL/well) can bind human NRP2-Fc, the EC50 of human NRP2-Fc is 0.1-0.5 μg/mL.
- Accession
- O60462-3
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M D M F P L T W V F L A L Y F S R H Q V R G Q P D P P C G G R L N S K D A G Y I T S P G Y P Q D Y P S H Q N C E W I V Y A P E P N Q K I V L N F N P H F E I E K H D C K Y D F I E I R D G D S E S A D L L G K H C G N I A P P T I I S S G S M L Y I K F T S D Y A R Q G A G F S L R Y E I F K T G S E D C S K N F T S P N G T I E S P G F P E K Y P H N L D C T F T I L A K P K M E I I L Q F L I F D L E H D P L Q V G E G D C K Y D W L D I W D G I P H V G P L I G K Y C G T K T P S E L R S S T G I L S L T F H T D M A V A K D G F S A R Y Y L V H Q E P L E N F Q C N V P L G M E S G R I A N E Q I S A S S T Y S D G R W T P Q Q S R L H G D D N G W T P N L D S N K E Y L Q V D L R F L T M L T A I A T Q G A I S R E T Q N G Y Y V K S Y K L E V S T N G E D W M V Y R H G K N H K V F Q A N N D A T E V V L N K L H A P L L T R F V R I R P Q T W H S G I A L R L E L F G C R V T D A P C S N M L G M L S G L I A D S Q I S A S S T Q E Y L W S P S A A R L V S S R S G W F P R I P Q A Q P G E E W L Q V D L G T P K T V K G V I I Q G A R G G D S I T A V E A R A F V R K F K V S Y S L N G K D W E Y I Q D P R T Q Q P K L F E G N M H Y D T P D I R R F D P I P A Q Y V R V Y P E R W S P A G I G M R L E V L G C D W T D S K P T V E T L G P T V K S E E T T T P Y P T E E E A T E C G E N C S F E D D K D L Q L P S G F N C N F D F L E E P C G W M Y D H A K W L R T T W A S S S S P N D R T F P D D R N F L R L Q S D S Q R E G Q Y A R L I S P P V H L P R S P V C M E F Q Y Q A T G G R G V A L Q V V R E A S Q E S K L L W V I R E D Q G G E W K H G R I I L P S Y D M E Y Q I V F E G V I G K G R S G E I A I D D I R I S T D V P L E N C M E P I S A F A V D I P E I H E R E G Y E D E I D D E Y E V D W S N S S S A T S G S G A P S T D K E K S W L Y
- Accession
- O60462
- Protein length
- Fragment
- Predicted molecular weight
- 120.7 kDa
- Amino acids
- 1 to 855
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.
- Sequence similarities
Belongs to the neuropilin family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NRP2 also known as Neuropilin-2 is a receptor protein involved in developmental processes including axon guidance and angiogenesis. The molecular mass of NRP2 is approximately 120 kDa. It is expressed in various tissues such as the nervous system and the vascular system but its expression can also be found in certain immune cells. NRP2 plays an important role in cellular signaling by binding to semaphorins and vascular endothelial growth factors (VEGF).
- Biological function summary
NRP2 influences several processes including axonal guidance and the formation of blood vessels. It acts as a co-receptor by forming complexes with other proteins to mediate cellular responses. This role in signaling helps guide neuron growth and regulates vascular development. The NRP2-associated complexes often modulate responses to external signals contributing to the precise control of network formation in tissues.
- Pathways
NRP2 plays a significant role in the VEGF signaling pathway and the semaphorin signaling pathway. These pathways are essential for cell growth migration and differentiation. NRP2 works closely with VEGF receptors like VEGFR2 to promote angiogenic responses. It also interacts with semaphorin family proteins to guide neuronal development. Together these pathways ensure the proper formation and function of neural and vascular networks.
- Associated diseases and disorders
NRP2 is connected to cancer progression and cardiovascular diseases. Altered expression of NRP2 has been observed in various cancer types where it may contribute to tumor growth and metastasis. In these diseases NRP2's interaction with its partners such as VEGFR2 becomes dysregulated affecting normal cellular processes. Cardiovascular conditions can also arise from issues in NRP2-related signaling where the normal function of blood vessel formation is compromised.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Recombinant human NRP2 protein (Fc Chimera) (ab276305)
Measured by its binding ability in a functional ELISA. Immobilized human VEGFC-His at 10 μg/mL (100 μL/well) can bind human NRP2-Fc, the EC50 of human NRP2-Fc is 0.1-0.5 μg/mL.
SDS-PAGE - Recombinant human NRP2 protein (Fc Chimera) (ab276305)
SDS-PAGE analysis of ab276305
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com