Recombinant Human NSDHL protein is a Human Full Length protein, in the 1 to 373 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M E P A V S E P M R D Q V A R T H L T E D T P K V N A D I E K V N Q N Q A K R C T V I G G S G F L G Q H M V E Q L L A R G Y A V N V F D I Q Q G F D N P Q V R F F L G D L C S R Q D L Y P A L K G V N T V F H C A S P P P S S N N K E L F Y R V N Y I G T K N V I E T C K E A G V Q K L I L T S S A S V I F E G V D I K N G T E D L P Y A M K P I D Y Y T E T K I L Q E R A V L G A N D P E K N F L T T A I R P H G I F G P R D P Q L V P I L I E A A R N G K M K F V I G N G K N L V D F T F V E N V V H G H I L A A E Q L S R D S T L G G K A F H I T N D E P I P F W T F L S R I L T G L N Y E A P K Y H I P Y W V A Y Y L A L L L S L L V M V I S P V I Q L Q P T F T P M R V A L A G T F H Y Y S C E R A K K A M G Y Q P L V T M D D A M E R T V Q S F R H L R R V K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity). Also plays a role in the regulation of the endocytic trafficking of EGFR (By similarity).
Alternative names
H105E3, NSDHL, Protein H105e3
Recommended products
Recombinant Human NSDHL protein is a Human Full Length protein, in the 1 to 373 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q15738-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E P A V S E P M R D Q V A R T H L T E D T P K V N A D I E K V N Q N Q A K R C T V I G G S G F L G Q H M V E Q L L A R G Y A V N V F D I Q Q G F D N P Q V R F F L G D L C S R Q D L Y P A L K G V N T V F H C A S P P P S S N N K E L F Y R V N Y I G T K N V I E T C K E A G V Q K L I L T S S A S V I F E G V D I K N G T E D L P Y A M K P I D Y Y T E T K I L Q E R A V L G A N D P E K N F L T T A I R P H G I F G P R D P Q L V P I L I E A A R N G K M K F V I G N G K N L V D F T F V E N V V H G H I L A A E Q L S R D S T L G G K A F H I T N D E P I P F W T F L S R I L T G L N Y E A P K Y H I P Y W V A Y Y L A L L L S L L V M V I S P V I Q L Q P T F T P M R V A L A G T F H Y Y S C E R A K K A M G Y Q P L V T M D D A M E R T V Q S F R H L R R V K
- Accession
- Q15738
- Protein length
- Full Length
- Amino acids
- 1 to 373
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity). Also plays a role in the regulation of the endocytic trafficking of EGFR (By similarity).
- Sequence similarities
Belongs to the 3-beta-HSD family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NSDHL also known as NAD(P)H steroid dehydrogenase-like is an enzyme linked to the cholesterol biosynthesis pathway. It has a molecular weight of approximately 41 kDa. This enzyme localizes to the endoplasmic reticulum membrane and the peroxisomes. NSDHL shows expression in various tissues with higher levels in the liver adrenal glands and the brain. This distribution highlights its essential role in metabolic processes across different tissue types.
- Biological function summary
NSDHL facilitates the removal of one hydrogen from NAD(P)H and adds it to the steroid precursor lathosterol an important intermediate in cholesterol biosynthesis. Although not part of a larger complex NSDHL performs significant enzymatic steps necessary for converting sterol intermediates. Its activity directly influences sterol composition vital for cell membrane integrity signaling and hormone synthesis.
- Pathways
The enzyme plays a critical role in the cholesterol biosynthesis pathway specifically affecting the conversion of lanosterol into cholesterol. NSDHL operates in tandem with other enzymes like DHCR7 influencing the downstream process of sterol maturation. The precise coordination of these enzymes ensures effective cholesterol production essential for maintaining cellular cholesterol levels and systemic lipid homeostasis.
- Associated diseases and disorders
Malfunctions in NSDHL can lead to disorders like CHILD syndrome (Congenital Hemidysplasia with Ichthyosiform erythroderma and Limb Defects) and CK syndrome (Conradi-Hünermann-Happle syndrome). These conditions link to cholesterol biosynthesis disruption impacting skin and skeletal development. NSDHL interacts with the EBP protein in these pathways suggesting a relationship between enzyme activity and phenotypic expression in these disorders. Understanding NSDHL's function and regulation is key to recognizing its involvement in metabolic syndromes and potential therapeutic targets.
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1 product image
SDS-PAGE - Recombinant Human NSDHL protein (ab162360)
ab162360 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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