Recombinant Human NSE protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, WB.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- Yes
Amino acid sequence
M S I E K I W A R E I L D S R G N P T V E V D L Y T A K G L F R A A V P S G A S T G I Y E A L E L R D G D K Q R Y L G K G V L K A V D H I N S T I A P A L I S S G L S V V E Q E K L D N L M L E L D G T E N K S K F G A N A I L G V S L A V C K A G A A E R E L P L Y R H I A Q L A G N S D L I L P V P A F N V I N G G S H A G N K L A M Q E F M I L P V G A E S F R D A M R L G A E V Y H T L K G V I K D K Y G K D A T N V G D E G G F A P N I L E N S E A L E L V K E A I D K A G Y T E K I V I G M D V A A S E F Y R D G K Y D L D F K S P T D P S R Y I T G D Q L G A L Y Q D F V R D Y P V V S I E D P F D Q D D W A A W S K F T A N V G I Q I V G D D L T V T N P K R I E R A V E E K A C N C L L L K V N Q I G S V T E A I Q A C K L A Q E N G W G V M V S H R S G E T E D T F I A D L V V G L C T G Q I K T G A P C R S E R L A K Y N Q L M R I E E E L G D E A R F A G H N F R N P S V L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab78797 can be used as a WB positive control in conjunction with ab90473. |
Associated Products
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Target data
Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Alternative names
Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
Recommended products
Recombinant Human NSE protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- Yes
- Biological activity
- Specific activity is 27,743.1 pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.
- Accession
- P09104-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 0.75% Potassium chloride, 0.242% Tris, 0.06% Magnesium sulphate
Sequence info
Amino acid sequence
- M S I E K I W A R E I L D S R G N P T V E V D L Y T A K G L F R A A V P S G A S T G I Y E A L E L R D G D K Q R Y L G K G V L K A V D H I N S T I A P A L I S S G L S V V E Q E K L D N L M L E L D G T E N K S K F G A N A I L G V S L A V C K A G A A E R E L P L Y R H I A Q L A G N S D L I L P V P A F N V I N G G S H A G N K L A M Q E F M I L P V G A E S F R D A M R L G A E V Y H T L K G V I K D K Y G K D A T N V G D E G G F A P N I L E N S E A L E L V K E A I D K A G Y T E K I V I G M D V A A S E F Y R D G K Y D L D F K S P T D P S R Y I T G D Q L G A L Y Q D F V R D Y P V V S I E D P F D Q D D W A A W S K F T A N V G I Q I V G D D L T V T N P K R I E R A V E E K A C N C L L L K V N Q I G S V T E A I Q A C K L A Q E N G W G V M V S H R S G E T E D T F I A D L V V G L C T G Q I K T G A P C R S E R L A K Y N Q L M R I E E E L G D E A R F A G H N F R N P S V L
- Accession
- P09104
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purity is > 98% by SDS PAGE. ab78797 is purified using conventional chromatography techniques.
General info
- Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
- Sequence similarities
Belongs to the enolase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.
- Biological function summary
NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.
- Pathways
NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.
- Associated diseases and disorders
NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.
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2 product images
Western blot - Recombinant Human NSE protein (ab78797)
All lanes: Anti-NSE antibody (ab90473) at 1 µg/mL
All lanes: Western blot - Recombinant Human NSE protein (ab78797) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 4min
SDS-PAGE - Recombinant Human NSE protein (ab78797)
SDS-PAGE analysis of ab78797 (3µg).
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