Recombinant human NT-4 protein (Active) is a Human Full Length protein, in the 81 to 210 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M G V S E T A P A S R R G E L A V C D A V S G W V T D R R T A V D L R G R E V E V L G E V P A A G G S P L R Q Y F F E T R C K A D N A E E G G P G A G G G G C R G V D R R H W V S E C K A K Q S Y V R A L T A D A Q G R V G W R W I R I D T A C V C T L L S R T G R A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Target-derived survival factor for peripheral sensory sympathetic neurons (PubMed:1742028). May promote ameloblast differentiation and subsequent reduction in proliferation of ameloblasts (By similarity).
Alternative names
NTF5, NTF4, Neurotrophin-4, NT-4, Neurotrophin-5, Neutrophic factor 4, NT-5
Recommended products
Recombinant human NT-4 protein (Active) is a Human Full Length protein, in the 81 to 210 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Rat C6 cell proliferation.
- Accession
- P34130-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.1% Trifluoroacetic acid
Sequence info
Amino acid sequence
- M G V S E T A P A S R R G E L A V C D A V S G W V T D R R T A V D L R G R E V E V L G E V P A A G G S P L R Q Y F F E T R C K A D N A E E G G P G A G G G G C R G V D R R H W V S E C K A K Q S Y V R A L T A D A Q G R V G W R W I R I D T A C V C T L L S R T G R A
- Accession
- P34130
- Protein length
- Full Length
- Predicted molecular weight
- 14.1 kDa
- Amino acids
- 81 to 210
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Target-derived survival factor for peripheral sensory sympathetic neurons (PubMed:1742028). May promote ameloblast differentiation and subsequent reduction in proliferation of ameloblasts (By similarity).
- Sequence similarities
Belongs to the NGF-beta family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NT-4 also known as Neurotrophin-4 is a member of the neurotrophin family of proteins. It has a molecular mass of around 14 kDa. NT-4 is expressed in both the central and peripheral nervous systems with significant presence in the brain particularly in regions such as the cortex and hippocampus. This protein acts through binding to the TrkB receptor and plays a direct role in supporting neuronal survival and differentiation.
- Biological function summary
NT-4 regulates the growth and maturation of neurons. It is a part of the neurotrophin signaling complex which includes other family members like brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). By promoting axonal growth and synaptic plasticity NT-4 influences both embryonic neurodevelopment and adult neural adaptations. Its functions are important for maintaining the health of neurons and overall brain function.
- Pathways
NT-4 is involved in the neurotrophin signaling pathway and MAPK pathway. It interacts with the TrkB receptor to activate signaling cascades that regulate neuronal survival and plasticity. In these pathways proteins like PIK3CA and MAPK1 interact with NT-4 facilitating its role in signal transduction that influences neural activities and responses to external stimuli.
- Associated diseases and disorders
NT-4 is linked to neurological conditions such as Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In Alzheimer's disease NT-4 in association with BDNF influences neuronal survival and synaptic maintenance which are often compromised as the disease progresses. In ALS interactions between NT-4 and TrkB receptors may affect motor neuron health and survival therefore playing a role in disease pathology. Understanding these relationships can shed light on potential therapeutic strategies.
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1 product image
SDS-PAGE - Recombinant human NT-4 protein (Active) (ab256094)
SDS-PAGE analysis of ab256094 (1 μg) under non-reducing (Lane 1) and reducing (Lane 2) conditions.
4-20% Tris-Glycine gel. Coomassie Blue staining.
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