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AB116147

Recombinant Human NT5C2 protein (His tag N-Terminus)

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Recombinant Human NT5C2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 561 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

NT5B, NT5CP, PNT5, NT5C2, Cytosolic purine 5'-nucleotidase, Cytosolic 5'-nucleotidase II, Cytosolic IMP/GMP-specific 5'-nucleotidase, Cytosolic nucleoside phosphotransferase 5'N, High Km 5'-nucleotidase, cN-II

1 Images
SDS-PAGE - Recombinant Human NT5C2 protein (His tag N-Terminus) (AB116147)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NT5C2 protein (His tag N-Terminus) (AB116147)

15% SDS-PAGE showing ab116147 (3 µg) at approximately 67.1 kDa.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P49902

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEEE","proteinLength":"Full Length","predictedMolecularWeight":"67.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":561,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P49902","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NT5C2 protein also known as cytosolic 5'-nucleotidase II plays an important role in nucleotide metabolism. It has a molecular mass of approximately 65 kDa. Researchers observe its expression in various tissues with higher levels typically found in the liver heart and brain. NT5C2 controls the conversion of nucleoside monophosphates to nucleosides and inorganic phosphate acting to regulate intracellular nucleotide levels.
Biological function summary

NT5C2 influences purine metabolism specifically in the catabolism of nucleotides. It does not function as part of a larger protein complex. Instead NT5C2 plays its role independently within the cell. This enzymatic activity contributes to maintaining nucleotide balance essential for DNA and RNA synthesis and repair and affects overall cellular metabolism.

Pathways

NT5C2 appears important in purine and pyrimidine metabolism pathways. Within these it directly impacts salvage pathways where it catalyzes reactions that recycle nucleotides from nucleic acid degradation. Its activity is relevant to proteins like APRT and HPRT which also participate in purine salvage processes ensuring efficient nucleotide utilization.

NT5C2 has associations with certain leukemias and neurological conditions. Mutations and dysregulation in NT5C2 can lead to chemoresistance in acute lymphoblastic leukemia affecting treatment efficacy. Additionally its connection with purine metabolism links it to Lesch-Nyhan syndrome where dysfunctional purine salvage causes severe neurological and behavioral abnormalities. The interaction with proteins like HPRT in Lesch-Nyhan syndrome accentuates the metabolic disruption observed.
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Specifications

Form

Liquid

Additional notes

ab116147 was purified using conventional chromatography.

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General info

Function

Broad specificity cytosolic 5'-nucleotidase that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates (PubMed : 10092873, PubMed : 12907246, PubMed : 1659319, PubMed : 9371705). In addition, possesses a phosphotransferase activity by which it can transfer a phosphate from a donor nucleoside monophosphate to an acceptor nucleoside, preferably inosine, deoxyinosine and guanosine (PubMed : 1659319, PubMed : 9371705). Has the highest activities for IMP and GMP followed by dIMP, dGMP and XMP (PubMed : 10092873, PubMed : 12907246, PubMed : 1659319, PubMed : 9371705). Could also catalyze the transfer of phosphates from pyrimidine monophosphates but with lower efficiency (PubMed : 1659319, PubMed : 9371705). Through these activities regulates the purine nucleoside/nucleotide pools within the cell (PubMed : 10092873, PubMed : 12907246, PubMed : 1659319, PubMed : 9371705).

Sequence similarities

Belongs to the 5'(3')-deoxyribonucleotidase family.

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Product protocols

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Target data

Broad specificity cytosolic 5'-nucleotidase that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates (PubMed : 10092873, PubMed : 12907246, PubMed : 1659319, PubMed : 9371705). In addition, possesses a phosphotransferase activity by which it can transfer a phosphate from a donor nucleoside monophosphate to an acceptor nucleoside, preferably inosine, deoxyinosine and guanosine (PubMed : 1659319, PubMed : 9371705). Has the highest activities for IMP and GMP followed by dIMP, dGMP and XMP (PubMed : 10092873, PubMed : 12907246, PubMed : 1659319, PubMed : 9371705). Could also catalyze the transfer of phosphates from pyrimidine monophosphates but with lower efficiency (PubMed : 1659319, PubMed : 9371705). Through these activities regulates the purine nucleoside/nucleotide pools within the cell (PubMed : 10092873, PubMed : 12907246, PubMed : 1659319, PubMed : 9371705).
See full target information NT5C2
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