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AB127374

Recombinant Human NTPDase 8 protein (His-DHFR tag N-Terminus)

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Recombinant Human NTPDase 8 protein (His-DHFR tag N-Terminus) is a Human Fragment protein, in the 305 to 458 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

UNQ2492/PRO5779, ENTPD8, Ectonucleoside triphosphate diphosphohydrolase 8, E-NTPDase 8, NTPDase 8, NTPDase8

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q5MY95

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Primary Antibodies

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Sample Prep & Detection Kits

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as ENTPD8.
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"16.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":458,"aminoAcidStart":305,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q5MY95","tags":[{"tag":"His-DHFR","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NTPDase 8 also known as ectonucleoside triphosphate diphosphohydrolase 8 is an enzyme with a molecular mass of approximately 65 kDa. Its main function is to hydrolyze nucleoside triphosphates and diphosphates to nucleoside monophosphates and inorganic phosphate. This reaction occurs on the extracellular surface where NTPDase 8 is located. The enzyme is expressed in various tissues including the liver and gastrointestinal tract contributing to the regulation of extracellular nucleotide levels.
Biological function summary

The enzyme plays an important role in purinergic signaling by controlling concentrations of ATP and ADP in the extracellular environment. NTPDase 8 is not part of a complex but interacts widely in tissue-specific manners. Its activity influences important physiological processes such as inflammation and thromboregulation. By modulating nucleotide levels it affects the signaling pathways involved in these biological processes having a direct impact on cellular communication and homeostasis.

Pathways

NTPDase 8 features prominently in the purinergic signaling pathway and the adenosine triphosphate (ATP) hydrolysis pathway. It is related to other NTPDase family members like NTPDase 1 and NTPDase 2 through its shared role in these pathways. NTPDase 8's enzymatic actions reduce extracellular ATP levels and correspondingly raise adenosine concentrations influencing signaling and responses within the cell particularly in immune and nervous system pathways.

NTPDase 8 is associated with conditions such as chronic inflammatory diseases and certain liver disorders. Its regulation of ATP and ADP levels can contribute to pathological states if dysregulated. The enzyme's activity relates to other proteins involved in these diseases such as ecto-5'-nucleotidase which together can impact purinergic signaling balance and possibly exacerbate inflammatory responses or hepatic function disruptions.
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Specifications

Form

Lyophilized

Additional notes

Purified via His tag

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General info

Function

Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity (PubMed : 17095758). Catalyzes the hydrolysis of nucleoside triphosphates (NTPs) and diphosphates (NDPs) (PubMed : 16752921, PubMed : 17095758, PubMed : 17603550). The enzyme sequentially removes phosphate groups in two successive steps, converting NTPs to nucleoside monophosphates (NMPs) via NDP intermediates (PubMed : 16752921, PubMed : 17095758, PubMed : 17603550). This activity contributes to the regulation of extracellular levels of nucleotides (PubMed : 16752921, PubMed : 17095758, PubMed : 17603550). Hydrolyzes ATP, UTP, ADP, and UDP and prefers triphosphonucleosides and adenine over uracil as substrates (PubMed : 17095758). Does not hydrolyzes AMP (PubMed : 17095758).

Sequence similarities

Belongs to the GDA1/CD39 NTPase family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity (PubMed : 17095758). Catalyzes the hydrolysis of nucleoside triphosphates (NTPs) and diphosphates (NDPs) (PubMed : 16752921, PubMed : 17095758, PubMed : 17603550). The enzyme sequentially removes phosphate groups in two successive steps, converting NTPs to nucleoside monophosphates (NMPs) via NDP intermediates (PubMed : 16752921, PubMed : 17095758, PubMed : 17603550). This activity contributes to the regulation of extracellular levels of nucleotides (PubMed : 16752921, PubMed : 17095758, PubMed : 17603550). Hydrolyzes ATP, UTP, ADP, and UDP and prefers triphosphonucleosides and adenine over uracil as substrates (PubMed : 17095758). Does not hydrolyzes AMP (PubMed : 17095758).
See full target information ENTPD8
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