Recombinant Human NUBP2 protein is a Human Full Length protein, in the 1 to 271 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M E A A A E P G N L A G V R H I I L V L S G K G G V G K S T I S T E L A L A L R H A G K K V G I L D V D L C G P S I P R M L G A Q G R A V H Q C D R G W A P V F L D R E Q S I S L M S V G F L L E K P D E A V V W R G P K K N A L I K Q F V S D V A W G E L D Y L V V D T P P G T S D E H M A T I E A L R P Y Q P L G A L V V T T P Q A V S V G D V R R E L T F C R K T G L R V M G I V E N M S G F T C P H C T E C T S V F S R G G G E E L A Q L A G V P F L G S V P L D P A L M R T L E E G H D F I Q E F P G S P A F A A L T S I A Q K I L D A T P A C L P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.
Alternative names
Cytosolic Fe-S cluster assembly factor NUBP2, Nucleotide-binding protein 2, NBP 2, NUBP2
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Recombinant Human NUBP2 protein is a Human Full Length protein, in the 1 to 271 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9Y5Y2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M E A A A E P G N L A G V R H I I L V L S G K G G V G K S T I S T E L A L A L R H A G K K V G I L D V D L C G P S I P R M L G A Q G R A V H Q C D R G W A P V F L D R E Q S I S L M S V G F L L E K P D E A V V W R G P K K N A L I K Q F V S D V A W G E L D Y L V V D T P P G T S D E H M A T I E A L R P Y Q P L G A L V V T T P Q A V S V G D V R R E L T F C R K T G L R V M G I V E N M S G F T C P H C T E C T S V F S R G G G E E L A Q L A G V P F L G S V P L D P A L M R T L E E G H D F I Q E F P G S P A F A A L T S I A Q K I L D A T P A C L P
- Accession
- Q9Y5Y2
- Protein length
- Full Length
- Predicted molecular weight
- 31 kDa
- Amino acids
- 1 to 271
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab187482 was purified using conventional chromatography.
General info
- Function
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.
- Sequence similarities
Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.
- Subcellular localisation
- Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome, Centriole
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NUBP2 also known as nucleotide-binding protein 2 functions as an ATPase and plays a role in cellular iron-sulfur cluster assembly. This protein has a mass of approximately 32 kDa. NUBP2 localizes in both the cytoplasm and nucleus indicating a dynamic cellular distribution. Its expression occurs in various tissues but it shows significant levels in highly proliferative tissues suggesting a link to cellular growth and metabolism.
- Biological function summary
NUBP2 associates with the cytosolic iron-sulfur assembly (CIA) complex. This complex is essential for the maturation of iron-sulfur clusters which are important for the function of a wide range of enzymes. By contributing to this maturation NUBP2 supports enzymatic activities that are necessary for the stability and function of proteins involved in DNA synthesis and repair as well as mitochondrial energy metabolism.
- Pathways
NUBP2 participates in the cytosolic iron-sulfur protein assembly pathway. This pathway is critical for biogenesis of iron-sulfur clusters used in various cellular processes. NUBP2 functions alongside other CIA components such as NUBP1 and CIAPIN1. By facilitating the transfer of iron-sulfur clusters to target apoproteins NUBP2 ensures the proper function of enzymes involved in processes like DNA repair and metabolic regulation.
- Associated diseases and disorders
Disruptions in NUBP2 function link to certain types of cancer and neurological disorders. Aberrant expression or mutations in NUBP2 may contribute to tumorigenesis as the proteins reliant on iron-sulfur clusters play pivotal roles in cell cycle regulation. Additionally defects in NUBP2 and its interacting partners such as NUBP1 may result in neurological impairments because of the important role of iron-sulfur clusters in neuronal function and maintenance.
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SDS-PAGE - Recombinant Human NUBP2 protein (ab187482)
15% SDS-PAGE analysis of ab187482 (3 μg)
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