JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB187482

Recombinant Human NUBP2 protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human NUBP2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 271 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Cytosolic Fe-S cluster assembly factor NUBP2, Nucleotide-binding protein 2, NBP 2, NUBP2

1 Images
SDS-PAGE - Recombinant Human NUBP2 protein (His tag N-Terminus) (AB187482)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NUBP2 protein (His tag N-Terminus) (AB187482)

15% SDS-PAGE analysis of ab187482 (3 μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9Y5Y2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMEAAAEPGNLAGVRHIILVLSGKGGVGKSTISTELALALRHAGKKVGILDVDLCGPSIPRMLGAQGRAVHQCDRGWAPVFLDREQSISLMSVGFLLEKPDEAVVWRGPKKNALIKQFVSDVAWGELDYLVVDTPPGTSDEHMATIEALRPYQPLGALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHCTECTSVFSRGGGEELAQLAGVPFLGSVPLDPALMRTLEEGHDFIQEFPGSPAFAALTSIAQKILDATPACLP","proteinLength":"Full Length","predictedMolecularWeight":"31 kDa","actualMolecularWeight":null,"aminoAcidEnd":271,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y5Y2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUBP2 also known as nucleotide-binding protein 2 functions as an ATPase and plays a role in cellular iron-sulfur cluster assembly. This protein has a mass of approximately 32 kDa. NUBP2 localizes in both the cytoplasm and nucleus indicating a dynamic cellular distribution. Its expression occurs in various tissues but it shows significant levels in highly proliferative tissues suggesting a link to cellular growth and metabolism.
Biological function summary

NUBP2 associates with the cytosolic iron-sulfur assembly (CIA) complex. This complex is essential for the maturation of iron-sulfur clusters which are important for the function of a wide range of enzymes. By contributing to this maturation NUBP2 supports enzymatic activities that are necessary for the stability and function of proteins involved in DNA synthesis and repair as well as mitochondrial energy metabolism.

Pathways

NUBP2 participates in the cytosolic iron-sulfur protein assembly pathway. This pathway is critical for biogenesis of iron-sulfur clusters used in various cellular processes. NUBP2 functions alongside other CIA components such as NUBP1 and CIAPIN1. By facilitating the transfer of iron-sulfur clusters to target apoproteins NUBP2 ensures the proper function of enzymes involved in processes like DNA repair and metabolic regulation.

Disruptions in NUBP2 function link to certain types of cancer and neurological disorders. Aberrant expression or mutations in NUBP2 may contribute to tumorigenesis as the proteins reliant on iron-sulfur clusters play pivotal roles in cell cycle regulation. Additionally defects in NUBP2 and its interacting partners such as NUBP1 may result in neurological impairments because of the important role of iron-sulfur clusters in neuronal function and maintenance.
style
left-column

Specifications

Form

Liquid

Additional notes

ab187482 was purified using conventional chromatography.

style
left-column

General info

Function

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.

Sequence similarities

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.
See full target information Cytosolic Fe-S cluster assembly factor NUBP2
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB179524

Anti-PKC eta antibody [EPR18513]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-PKC eta antibody [EPR18513] (AB179524)

  • 5
  • 1
Primary Antibodies

AB182126

Anti-PKC delta antibody [EPR17075]

KO Validated|RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PKC delta antibody [EPR17075] (AB182126)

  • 4
  • 7
Primary Antibodies

AB108970

Anti-PKC zeta antibody [EP1490(2)]

RabMAb|KO Validated

Western blot - Anti-PKC zeta antibody [EP1490(2)] (AB108970)

  • 5
  • 1
Proteins & Peptides

AB60849

Recombinant human PKC eta protein

Western blot - Recombinant human PKC eta protein (AB60849)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com