Recombinant Human Nuclear respiratory factor 2 protein (His tag) is a Human Full Length protein, in the 1 to 395 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, ELISA, WB, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, ELISA, WB, MS
- Biologically active
- No
Amino acid sequence
M S L V D L G K K L L E A A R A G Q D D E V R I L M A N G A P F T T D W L G T S P L H L A A Q Y G H Y S T T E V L L R A G V S R D A R T K V D R T P L H M A A S E G H A S I V E V L L K H G A D V N A K D M L K M T A L H W A T E H N H Q E V V E L L I K Y G A D V H T Q S K F C K T A F D I S I D N G N E D L A E I L Q I A M Q N Q I N T N P E S P D T V T I H A A T P Q F I I G P G G V V N L T G L V S S E N S S K A T D E T G V S A V Q F G N S S T S V L A T L A A L A E A S A P L S N S S E T P V V A T E E V V T A E S V D G A I Q Q V V S S G G Q Q V I T I V T D G I Q L G N L H S I P T S G I G Q P I I V T M P D G Q Q V L T V P A T D I A E E T V I S E E P P A K R Q C I E I I E N R V E S A E I E E R E A L Q K Q L D E A N R E A Q K Y R Q Q L L K K E Q E A E A Y R Q K L E A M T R L Q T N K E A V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcription factor capable of interacting with purine rich repeats (GA repeats) (PubMed:10675337, PubMed:8441384, PubMed:8816484). Acts as a master regulator of nuclear-encoded mitochondrial genes (By similarity). (Microbial infection) Necessary for the expression of the Adenovirus E4 gene.
Alternative names
E4TF1B, GABPB, GABPB2, GABPB1, GA-binding protein subunit beta-1, GABP subunit beta-1, GABPB-1, GABP subunit beta-2, Nuclear respiratory factor 2, Transcription factor E4TF1-47, Transcription factor E4TF1-53, GABPB-2
Recommended products
Recombinant Human Nuclear respiratory factor 2 protein (His tag) is a Human Full Length protein, in the 1 to 395 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, ELISA, WB, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, ELISA, WB, MS
- Biologically active
- No
- Accession
- Q06547-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 3.4% Imidazole
Constituents: 2.9% Sodium chloride, 0.24% Tris
Sequence info
Amino acid sequence
- M S L V D L G K K L L E A A R A G Q D D E V R I L M A N G A P F T T D W L G T S P L H L A A Q Y G H Y S T T E V L L R A G V S R D A R T K V D R T P L H M A A S E G H A S I V E V L L K H G A D V N A K D M L K M T A L H W A T E H N H Q E V V E L L I K Y G A D V H T Q S K F C K T A F D I S I D N G N E D L A E I L Q I A M Q N Q I N T N P E S P D T V T I H A A T P Q F I I G P G G V V N L T G L V S S E N S S K A T D E T G V S A V Q F G N S S T S V L A T L A A L A E A S A P L S N S S E T P V V A T E E V V T A E S V D G A I Q Q V V S S G G Q Q V I T I V T D G I Q L G N L H S I P T S G I G Q P I I V T M P D G Q Q V L T V P A T D I A E E T V I S E E P P A K R Q C I E I I E N R V E S A E I E E R E A L Q K Q L D E A N R E A Q K Y R Q Q L L K K E Q E A E A Y R Q K L E A M T R L Q T N K E A V
- Accession
- Q06547
- Protein length
- Full Length
- Predicted molecular weight
- 43.31 kDa
- Amino acids
- 1 to 395
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab204765 was purified by 6x His tag / Immobilized-metal affinity chromatography (IMAC).
General info
- Function
Transcription factor capable of interacting with purine rich repeats (GA repeats) (PubMed:10675337, PubMed:8441384, PubMed:8816484). Acts as a master regulator of nuclear-encoded mitochondrial genes (By similarity).
- Post-translational modifications
Acetylated by EP300/p300. Deacetylated by SIRT7, promoting heterotetramerization and activity.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as GABPB2
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nuclear respiratory factor 2 (NRF2) also known as GABP (GA-binding protein) is a transcription factor that regulates the expression of genes involved in cellular energy metabolism and mitochondrial biogenesis. NRF2 is composed of several subunits with GABPA as its DNA-binding subunit having a molecular mass approximately of 50 kDa. This protein is expressed in various tissues with higher concentrations found in metabolic active tissues such as the brain and muscle. It plays a significant role in controlling the transcription of genes tied to mitochondrial function and overall cellular energy homeostasis.
- Biological function summary
NRF2 interacts with other protein factors to initiate and regulate transcription. It functions as part of the GA-binding protein complex interacting with other transcription factors to control gene expression. This regulatory mechanism is vital for cellular adaptation to changing energy demands and influences oxidative stress responses. NRF2 is actively involved in the induction of antioxidant enzymes and proteins that protect the cell from oxidative damage contributing to cellular longevity and function.
- Pathways
NRF2 plays an important role in the oxidative stress response pathway and mitochondrial biogenesis. It interacts with other proteins like KEAP1 which regulates NRF2 stability and activity. In the oxidative stress response NRF2 translocates to the nucleus to activate genes associated with antioxidant responses whereas in mitochondrial biogenesis it influences the expression of genes involved in mitochondrial replication and energy production. These pathways are critical for maintaining cellular health and energy supply in response to metabolic demands.
- Associated diseases and disorders
NRF2 has links to neurodegenerative conditions and certain cancer types. In neurodegenerative diseases such as Alzheimer's impaired NRF2 function can lead to increased oxidative stress and neuronal damage. Altered NRF2 signaling is also implicated in some cancers where its role in regulating cell growth and survival becomes dysregulated. NRF2's interaction with KEAP1 is significant in both areas; mutations or changes in KEAP1 can affect NRF2 levels and activity contributing to disease progression and resistance to therapies.
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1 product image
SDS-PAGE - Recombinant Human Nuclear respiratory factor 2 protein (His tag) (ab204765)
10% SDS-PAGE analysis of ab204765 with a 6x His tag. The gel was stained with Coomassie Blue.
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