Recombinant Human Nucleosome protein (Biotin) (His tag N-Terminus)
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Recombinant Human Nucleosome protein (Biotin) (His tag N-Terminus) is a Human Full Length protein, in the 2 to 136 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.
View Alternative Names
H2BFQ, HIST2H2BE, H2BC21, Histone H2B type 2-E, H2B-clustered histone 21, Histone H2B-GL105, Histone H2B.q, H2B/q
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human Nucleosome protein (Biotin) (His tag N-Terminus) (AB216216)
SDS-PAGE analysis of ab216216.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human Nucleosome protein (Biotin) (His tag N-Terminus) (AB216216)
SDS-PAGE analysis of ab216216.
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Proteins & Peptides
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Recombinant human PRMT5 + MEP50 protein (Active) (DDDDK tag N-Terminus, His tag N-Terminus)
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Reactivity data
Product details
Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
ab216216 is Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A, H2B, H3 and H4. Each histone protein has an N-terminal His-tag. Each H2B has a C-terminal Cys added as a biotinylation site. Total histone MW (before biotinylation) = 113.8 kDa
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Nucleosomes organize and protect the DNA strand modulating access for DNA replication and transcription machinery. Nucleosomes are part of the larger chromatin complex and regulate gene expression by controlling DNA accessibility. Changes in histone modifications within nucleosomes alter chromatin's structure influencing the transcriptional activity of genes. This dynamic alteration is essential for cellular functions such as DNA repair replication and genome stability.
Pathways
Nucleosomes play central roles in the regulation of epigenetic pathways involved in gene expression and silencing. They interact with other proteins like histone-modifying enzymes and chromatin remodelers such as the Polycomb group proteins and the SWI/SNF complexes. These interactions impact pathways like the cell cycle control and DNA damage response coordinating cellular processes to maintain genomic integrity and respond to environmental signals.
Specifications
Form
Liquid
General info
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
Sequence similarities
Belongs to the histone H2B family.
Post-translational modifications
Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation (PubMed:12757711). Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).. ADP-ribosylated by PARP1 or PARP2 on Ser-7 (H2BS6ADPr) in response to DNA damage (PubMed:34874266). H2BS6ADPr promotes recruitment of CHD1L (PubMed:34874266). Mono-ADP-ribosylated on Glu-3 (H2BE2ADPr) by PARP3 in response to single-strand breaks (PubMed:27530147). Poly ADP-ribosylation on Glu-36 (H2BE35ADPr) by PARP1 regulates adipogenesis: it inhibits phosphorylation at Ser-37 (H2BS36ph), thereby blocking expression of pro-adipogenetic genes (By similarity).. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.
Subcellular localisation
Nucleus
Target data
Product promise
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