Recombinant Human NUDT2 protein is a Human Full Length protein, in the 1 to 147 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A L R A C G L I I F R R C L I P K V D N N A I E F L L L Q A S D G I H H W T P P K G H V E P G E D D L E T A L R E T Q E E A G I E A G Q L T I I E G F K R E L N Y V A R N K P K T V I Y W L A E V K D Y D V E I R L S H E H Q A Y R W L G L E E A C Q L A Q F K E M K A A L Q E G H Q F L C S I E A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the asymmetric hydrolysis of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to yield AMP and ATP (By similarity). Exhibits decapping activity towards FAD-capped RNAs and dpCoA-capped RNAs in vitro (By similarity).
Alternative names
APAH1, NUDT2, Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical], Nucleoside diphosphate-linked moiety X motif 2, Ap4A hydrolase, Ap4Aase, Diadenosine tetraphosphatase, Nudix motif 2
Recommended products
Recombinant Human NUDT2 protein is a Human Full Length protein, in the 1 to 147 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P50583-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.242% Tris, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A L R A C G L I I F R R C L I P K V D N N A I E F L L L Q A S D G I H H W T P P K G H V E P G E D D L E T A L R E T Q E E A G I E A G Q L T I I E G F K R E L N Y V A R N K P K T V I Y W L A E V K D Y D V E I R L S H E H Q A Y R W L G L E E A C Q L A Q F K E M K A A L Q E G H Q F L C S I E A
- Accession
- P50583
- Protein length
- Full Length
- Predicted molecular weight
- 19 kDa
- Amino acids
- 1 to 147
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab103048 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the asymmetric hydrolysis of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to yield AMP and ATP (By similarity). Exhibits decapping activity towards FAD-capped RNAs and dpCoA-capped RNAs in vitro (By similarity).
- Sequence similarities
Belongs to the Nudix hydrolase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein NUDT2 also known as Nucleoside Diphosphate-Linked Moiety X-Type Motif 2 is an enzyme that hydrolyzes nucleoside diphosphates. It has a molecular mass of approximately 22 kDa. This protein is expressed in various tissues including the brain liver and heart. NUDT2's mechanical function centers on its ability to regulate cellular nucleoside diphosphate levels by converting them into their monophosphate forms which is vital for the proper recycling of nucleotide pool components.
- Biological function summary
The protein plays a role in controlling intracellular hydrolysis of nucleoside diphosphates ensuring proper nucleotide balance. Although it operates independently it functions in synergy with other proteins involved in nucleotide metabolism. This precision in nucleotide regulation is essential for maintaining nucleic acid synthesis as well as energy transfer processes within the cell. NUDT2 does not form large complexes but interacts dynamically within metabolic hubs.
- Pathways
NUDT2 participates in nucleotide metabolism and salvage pathways. It contributes predominantly to pathways that recycle nucleotides essential for cellular replication and repair processes. Within these pathways NUDT2 closely interacts with enzymes such as nucleoside diphosphate kinases which play a complementary role in maintaining nucleotide balance. These interactions ensure efficient utilization of resources required for vital cellular functions.
- Associated diseases and disorders
NUDT2 has been linked to conditions such as cancer and metabolic disorders. Aberrant expression or dysfunction of NUDT2 can result in nucleotide imbalance leading to problems with cell proliferation or metabolic dysregulation. In certain cancers altered expression of NUDT2 in conjunction with disrupted functions of tumor suppressor proteins can contribute to uncontrolled cell growth. Understanding NUDT2's role in these diseases can guide therapeutic strategies aimed at restoring normal nucleotide metabolism.
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1 product image
SDS-PAGE - Recombinant Human NUDT2 protein (ab103048)
15% SDS-PAGE analysis of ab103048 (3μg)
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