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AB156348

Recombinant Human NUDT4 protein

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Recombinant Human NUDT4 protein is a Human Full Length protein, in the 1 to 180 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

DIPP2, KIAA0487, HDCMB47P, NUDT4, Diphosphoinositol polyphosphate phosphohydrolase 2, DIPP-2, Nucleoside diphosphate-linked moiety X motif 4, Nudix motif 4

1 Images
SDS-PAGE - Recombinant Human NUDT4 protein (AB156348)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NUDT4 protein (AB156348)

SDS-PAGE of ab156348 (3?g) under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9NZJ9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMMKFKPNQTRTYDREGFKKRAACLCFRSEQEDEVLLVSSSRYPDQWIVPGGGMEPEEEPGGAAVREVYEEAGVKGKLGRLLGIFENQDRKHRTYVYVLTVTEILEDWEDSVNIGRKREWFKVEDAIKVLQCHKPVHAEYLEKLKLGCSPANGNSTVPSLPDNNALFVTAAQTSGLPSSVR","proteinLength":"Full Length","predictedMolecularWeight":"22.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":180,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NZJ9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUDT4 also called Nudix hydrolase 4 is a member of the Nudix hydrolase family. This protein is approximately 22 kDa in mass. NUDT4 is an enzyme that hydrolyzes diadenosine polyphosphates and other substrates with a diphosphate bond at the N-terminal. It is expressed in various tissues with significant expression in the brain and testis. NUDT4's presence in these tissues suggests it plays roles in energy metabolism and cellular signaling.
Biological function summary

NUDT4 facilitates the regulation of nucleotide pool balance and the hydrolysis of altered nucleotides ensuring the maintenance of cellular homeostasis. It is not part of a larger complex acting independently to maintain nucleotide integrity. By controlling the levels of signaling molecules such as diadenosine tetraphosphate NUDT4 influences cellular processes like signal transduction and energy regulation.

Pathways

NUDT4 functions are vital in purine metabolism and nucleotide salvage pathways. It interacts with enzymes like NUDT5 and other related proteins to maintain nucleotide levels preventing the accumulation of potentially harmful derivatives. NUDT4 contributes to the control of molecules which are important in maintaining cellular signaling pathways.

NUDT4 has implications in neurodegenerative diseases and cancer. Its dysfunction or altered expression can disrupt nucleotide balance contributing to the pathophysiology of these conditions. Through its connection to pathways involving impaired signaling or cellular stress responses NUDT4's relationship with disorders like Alzheimer's disease and certain cancers reveals its potential as a target for therapeutic interventions aimed at restoring nucleotide homeostasis.
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Specifications

Form

Liquid

Additional notes

ab156348 was purified using conventional chromatography techniques.

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General info

Function

Cleaves the beta-phosphate from diphosphoinositol polyphosphates such as PP-InsP5 (diphosphoinositol pentakisphosphate), PP-InsP4 (diphosphoinositol tetrakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction (PubMed : 10777568). Diadenosine polyphosphates, particularly Ap6A (P(1),P(6)-bis(5a-adenosyl) hexaphosphate) and Ap5A (P(1),P(5)-bis(5'-adenosyl) pentaphosphate) are downstream effectors of a signaling cascade that regulates cardiac KATP channels, can also be substrates, although with lower preference than the diphosphoinositol polyphosphates (PubMed : 10777568). Can also catalyze the hydrolysis of 5-phosphoribose 1-diphosphate, generating the glycolytic activator ribose 1,5-bisphosphate (PubMed : 12370170). Does not play a role in U8 snoRNA decapping activity (By similarity). Binds U8 snoRNA (By similarity).

Sequence similarities

Belongs to the Nudix hydrolase family. DIPP subfamily.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaves the beta-phosphate from diphosphoinositol polyphosphates such as PP-InsP5 (diphosphoinositol pentakisphosphate), PP-InsP4 (diphosphoinositol tetrakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction (PubMed : 10777568). Diadenosine polyphosphates, particularly Ap6A (P(1),P(6)-bis(5a-adenosyl) hexaphosphate) and Ap5A (P(1),P(5)-bis(5'-adenosyl) pentaphosphate) are downstream effectors of a signaling cascade that regulates cardiac KATP channels, can also be substrates, although with lower preference than the diphosphoinositol polyphosphates (PubMed : 10777568). Can also catalyze the hydrolysis of 5-phosphoribose 1-diphosphate, generating the glycolytic activator ribose 1,5-bisphosphate (PubMed : 12370170). Does not play a role in U8 snoRNA decapping activity (By similarity). Binds U8 snoRNA (By similarity).
See full target information NUDT4
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Product promise

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For full details, please see our Terms & Conditions
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