Recombinant Human NUMB protein is a Human Full Length protein, in the 1 to 135 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M P Y P A P N V P V V G I T P S Q M V A N V F G T A G H P Q A A H P H Q S P S L V R Q Q T F P H Y E A S S A T T S P F F K P P A Q H L N G S A A F N G V D D G R L A S A D R H T E V P T G T C P V D P F E A Q W A A L E N K S K Q R T N P S P T N P F S S D L Q K T F E I E L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulates clathrin-mediated receptor endocytosis (PubMed:18657069). Plays a role in the process of neurogenesis (By similarity). Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate (By similarity). Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity (By similarity). May also mediate local repair of brain ventricular wall damage (By similarity).
Alternative names
C14orf41, NUMB, Protein numb homolog, h-Numb, Protein S171
Recommended products
Recombinant Human NUMB protein is a Human Full Length protein, in the 1 to 135 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P49757-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M P Y P A P N V P V V G I T P S Q M V A N V F G T A G H P Q A A H P H Q S P S L V R Q Q T F P H Y E A S S A T T S P F F K P P A Q H L N G S A A F N G V D D G R L A S A D R H T E V P T G T C P V D P F E A Q W A A L E N K S K Q R T N P S P T N P F S S D L Q K T F E I E L
- Accession
- P49757
- Protein length
- Full Length
- Predicted molecular weight
- 40.59 kDa
- Amino acids
- 1 to 135
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Regulates clathrin-mediated receptor endocytosis (PubMed:18657069). Plays a role in the process of neurogenesis (By similarity). Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate (By similarity). Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity (By similarity). May also mediate local repair of brain ventricular wall damage (By similarity).
- Post-translational modifications
Phosphorylated on Ser-276 and Ser-295 by CaMK1.
- Subcellular localisation
- Endosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NUMB also known as numb protein is a cytoplasmic protein involved in cell fate determination. It has an approximate molecular mass of 75 kDa. NUMB plays a critical role in asymmetric cell division particularly during neural development. The protein is expressed in various tissues including the brain heart and liver but most prominently in neural tissues. NUMB influences the timing of differentiation and proliferation by interacting with several cellular components.
- Biological function summary
NUMB proteins are important to regulating cell fate decisions. NUMB primarily functions as a part of a larger complex including proteins like Notch and Eps15. In neural stem cells NUMB acts as an inhibitor of the Notch signaling pathway. It also regulates endocytosis and the signaling pathways that contribute to maintaining cellular diversity during development. NUMB interactions influence processes like cell adhesion and migration which are essential for proper tissue formation.
- Pathways
NUMB proteins play significant roles within the Notch and Hedgehog signaling pathways. These are critical pathways for developmental processes. In the Notch pathway NUMB negatively regulates Notch receptor activity preventing Notch-mediated gene expression. NUMB interacts with other proteins such as alpha-adaptin and LNX1 through these pathways helping regulate the endocytic trafficking of key signaling molecules. In the Hedgehog pathway NUMB affects signal transduction though its role is less defined compared to its involvement in the Notch pathway.
- Associated diseases and disorders
NUMB proteins have been implicated in breast cancer and Alzheimer's disease. In breast cancer alterations in NUMB expression or function can contribute to tumor progression due to dysregulation of the Notch pathway. Its interaction with proteins like MDM2 influences the stability and activity of p53 an important tumor suppressor. In Alzheimer's disease NUMB's involvement in endocytic processes may impact amyloid precursor protein processing and beta-amyloid plaque formation which are central to the disease pathology.
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1 product image
SDS-PAGE - Recombinant Human NUMB protein (ab131949)
12.5% SDS-PAGE analysis of ab131949 stained with Coomassie Blue.
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