Recombinant Human NUR77 protein is a Human Full Length protein, in the 1 to 598 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M P C I Q A Q Y G T P A P S P G P R D H L A S D P L T P E F I K P T M D L A S P E A A P A A P T A L P S F S T F M D G Y T G E F D T F L Y Q L P G T V Q P C S S A S S S A S S T S S S S A T S P A S A S F K F E D F Q V Y G C Y P G P L S G P V D E A L S S S G S D Y Y G S P C S A P S P S T P S F Q P P Q L S P W D G S F G H F S P S Q T Y E G L R A W T E Q L P K A S G P P Q P P A F F S F S P P T G P S P S L A Q S P L K L F P S Q A T H Q L G E G E S Y S M P T A F P G L A P T S P H L E G S G I L D T P V T S T K A R S G A P G G S E G R C A V C G D N A S C Q H Y G V R T C E G C K G F F K R T V Q K N A K Y I C L A N K D C P V D K R R R N R C Q F C R F Q K C L A V G M V K E V V R T D S L K G R R G R L P S K P K Q P P D A S P A N L L T S L V R A H L D S G P S T A K L D Y S K F Q E L V L P H F G K E D A G D V Q Q F Y D L L S G S L E V I R K W A E K I P G F A E L S P A D Q D L L L E S A F L E L F I L R L A Y R S K P G E G K L I F C S G L V L H R L Q C A R G F G D W I D S I L A F S R S L H S L L V D V P A F A C L S A L V L I T D R H G L Q E P R R V E E L Q N R I A S C L K E H V A A V A G E P Q P A S C L S R L L G K L P E L R T L C T Q G L Q R I F Y L K L E D L V P P P P I I D K I F M D T L P F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' (PubMed:18690216, PubMed:8121493, PubMed:9315652). Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain (PubMed:18690216). Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (PubMed:22983157). Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA) (By similarity). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter (PubMed:15466594). May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration (By similarity). Plays a role in the vascular response to injury (By similarity). In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7.
Alternative names
GFRP1, HMR, NAK1, NR4A1, Nuclear receptor subfamily 4immunitygroup A member 1, Early response protein NAK1, Nuclear hormone receptor NUR/77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3, Nur77
Recommended products
Recombinant Human NUR77 protein is a Human Full Length protein, in the 1 to 598 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P22736-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M P C I Q A Q Y G T P A P S P G P R D H L A S D P L T P E F I K P T M D L A S P E A A P A A P T A L P S F S T F M D G Y T G E F D T F L Y Q L P G T V Q P C S S A S S S A S S T S S S S A T S P A S A S F K F E D F Q V Y G C Y P G P L S G P V D E A L S S S G S D Y Y G S P C S A P S P S T P S F Q P P Q L S P W D G S F G H F S P S Q T Y E G L R A W T E Q L P K A S G P P Q P P A F F S F S P P T G P S P S L A Q S P L K L F P S Q A T H Q L G E G E S Y S M P T A F P G L A P T S P H L E G S G I L D T P V T S T K A R S G A P G G S E G R C A V C G D N A S C Q H Y G V R T C E G C K G F F K R T V Q K N A K Y I C L A N K D C P V D K R R R N R C Q F C R F Q K C L A V G M V K E V V R T D S L K G R R G R L P S K P K Q P P D A S P A N L L T S L V R A H L D S G P S T A K L D Y S K F Q E L V L P H F G K E D A G D V Q Q F Y D L L S G S L E V I R K W A E K I P G F A E L S P A D Q D L L L E S A F L E L F I L R L A Y R S K P G E G K L I F C S G L V L H R L Q C A R G F G D W I D S I L A F S R S L H S L L V D V P A F A C L S A L V L I T D R H G L Q E P R R V E E L Q N R I A S C L K E H V A A V A G E P Q P A S C L S R L L G K L P E L R T L C T Q G L Q R I F Y L K L E D L V P P P P I I D K I F M D T L P F
- Accession
- P22736
- Protein length
- Full Length
- Predicted molecular weight
- 91.85 kDa
- Amino acids
- 1 to 598
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' (PubMed:18690216, PubMed:8121493, PubMed:9315652). Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain (PubMed:18690216). Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (PubMed:22983157). Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA) (By similarity). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter (PubMed:15466594). May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration (By similarity). Plays a role in the vascular response to injury (By similarity).
- Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
- Post-translational modifications
Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.
- Subcellular localisation
- Nucleus, Mitochondrion
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NUR77 also known as NR4A1 is an orphan nuclear receptor and transcription factor with a molecular mass of around 65 kDa. This protein plays an important role in mediating gene expression by binding DNA at specific response elements. It is expressed in various tissues including the thymus spleen and central nervous system. NUR77 achieves its mechanical function by regulating gene transcription involved in cell proliferation apoptosis and metabolism.
- Biological function summary
The functions of NUR77 impact multiple cellular processes that are essential for maintaining cellular homeostasis. As part of its biological roles NUR77 can form complexes with other nuclear receptors and influence gene expression. It is particularly significant in T-cell receptor signaling where it influences the balance between cell survival and cell death. This modulation helps in the regulation of immune functions and cellular development.
- Pathways
NUR77 integrates into the MAPK signaling and apoptosis pathways. It interacts with proteins such as Bcl-2 and p53 facilitating cellular responses to stress and developmental cues. In the MAPK pathway NUR77 can modulate transcriptional activities affecting cell proliferation and differentiation while in apoptotic pathways it contributes to the regulation of programmed cell death making it a critical modulator in these contexts.
- Associated diseases and disorders
NUR77 has been associated with cancer and atherosclerosis. Its role in apoptosis makes it a point of interest in cancer research where altered NUR77 expression can influence tumor growth and resistance to therapy. In the context of atherosclerosis aberrant activity of NUR77 affects lipid metabolism and inflammation. Through its link to pathways involving Bcl-2 and p53 NUR77 remains a significant target in both therapeutic research and disease management.
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1 product image
SDS-PAGE - Recombinant Human NUR77 protein (ab114315)
SDS-PAGE analysis of ab114315 on a 12.5% gel stained with Coomassie Blue.
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