JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB171488

Recombinant Human OBFC2A protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human OBFC2A protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 204 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

OBFC2A, SSB2, NABP1, SOSS complex subunit B2, Nucleic acid-binding protein 1, Oligonucleotide/oligosaccharide-binding fold-containing protein 2A, Sensor of single-strand DNA complex subunit B2, Sensor of ssDNA subunit B2, Single-stranded DNA-binding protein 2, SOSS-B2, hSSB2

1 Images
SDS-PAGE - Recombinant Human OBFC2A protein (His tag N-Terminus) (AB171488)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human OBFC2A protein (His tag N-Terminus) (AB171488)

15% SDS-PAGE analysis of 3µg ab171488.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q96AH0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMNRVNDPLIFIRDIKPGLKNLNVVFIVLEIGRVTKTKDGHEVRSCKVADKTGSITISVWDEIGGLIQPGDIIRLTRGYASMWKGCLTLYTGRGGELQKIGEFCMVYSEVPNFSEPNPDYRGQQNKGAQSEQKNNSMNSNMGTGTFGPVGNGVHTGPESREHQFSHAGRSNGRGLINPQLQGTASNQTVMTTISNGRDPRRAFKR","proteinLength":"Full Length","predictedMolecularWeight":"24.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":204,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96AH0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OBFC2A also known as hSSB2 is a single-stranded DNA-binding protein with a molecular mass of approximately 29 kDa. It expresses in several tissues including the brain heart and skeletal muscle. OBFC2A plays a role in protecting single-stranded DNA regions during DNA replication and repair. By binding to single-stranded DNA OBFC2A prevents unwanted DNA degradation and assists in maintaining genome stability.
Biological function summary

OBFC2A participates in DNA replication and repair processes. It forms part of the telomere maintenance complex where its function ensures effective telomere replication and elongation. This involvement helps preserve chromosomal integrity. By working within this complex OBFC2A contributes to the overall stability and maintenance of genetic information during cell division.

Pathways

OBFC2A is important in telomere maintenance and the DNA damage response pathways. In the telomere maintenance pathway OBFC2A interacts with proteins like POT1 and TPP1 which are part of the shelterin complex ensuring proper telomeric function. In the DNA damage response pathway OBFC2A works alongside proteins such as ATR contributing to the recognition and repair of damaged DNA signaling the appropriate cellular responses to maintain genetic fidelity.

OBFC2A has associations with cancer and telomere-related disorders. Its involvement in maintaining telomere integrity makes it relevant in cancer progression as mutations or dysregulation in OBFC2A could potentially lead to genomic instability and tumorigenesis. Furthermore its interaction with proteins like POT1 connects it to hereditary disorders such as dyskeratosis congenita where telomere maintenance is compromised resulting in premature cell aging and related pathologies.
style
left-column

Specifications

Form

Liquid

Additional notes

ab171488 was purified using conventional chromatography techniques.

style
left-column

General info

Function

Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.

Sequence similarities

Belongs to the SOSS-B family. SOSS-B2 subfamily.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.
See full target information NABP1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB236402

Anti-OBFC2A antibody [OTI6B10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (AB236402)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com