Recombinant human Oct4 protein
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(3 Publications)
Recombinant human Oct4 protein is a Human Full Length protein, in the 1 to 360 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.
View Alternative Names
OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.
Pathways
Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.
Specifications
Form
Liquid
General info
Function
The protein expressed by the gene POU5F1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and forms a trimeric complex with SOX2 or SOX15 on DNA. It controls the expression of several genes involved in embryonic development, including YES1, FGF4, UTF1, and ZFP206. This protein is critical for early embryogenesis and for maintaining embryonic stem cell pluripotency. This supplementary information is collated from multiple sources and compiled automatically.
Sequence similarities
Belongs to the POU transcription factor family. Class-5 subfamily.
Post-translational modifications
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
Subcellular localisation
Nucleus
Target data
Publications (3)
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Cell reports 39:110919 PubMed35675767
2022
Applications
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Species
Unspecified reactive species
Nature communications 12:3337 PubMed34099689
2021
Applications
Unspecified application
Species
Unspecified reactive species
Cell reports 30:1478-1490.e6 PubMed32023463
2020
Applications
Unspecified application
Species
Unspecified reactive species
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