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AB98249

Recombinant Human Ogg1 protein

1

(1 Review)

|

(5 Publications)

Recombinant Human Ogg1 protein is a Human Full Length protein, in the 1 to 345 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

MMH, MUTM, OGH1, OGG1, N-glycosylase/DNA lyase

1 Images
Western blot - Recombinant Human Ogg1 protein (AB98249)
  • WB

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Western blot - Recombinant Human Ogg1 protein (AB98249)

15% SDS-PAGE analysis of 3μg ab98249

All lanes:

Western blot - Recombinant Human Ogg1 protein (ab98249)

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Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O15527

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHTGSMPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG","proteinLength":"Full Length","predictedMolecularWeight":"41.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":345,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O15527","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ogg1 protein also known as 8-oxoguanine DNA glycosylase or Oggone is an important repair enzyme responsible for the removal of oxidatively damaged guanine bases from DNA. This protein has a molecular mass of approximately 39 kDa and is expressed in various tissues including the brain liver and lungs. Ogg1 is significant in maintaining genome integrity by excising 8-oxoguanine lesions which can otherwise lead to mutations if left unrepaired.
Biological function summary

Within cellular processes Ogg1 functions primarily to maintain DNA stability by participating in the base excision repair pathway. This protein operates as part of a complex that identifies and excises damaged bases subsequently facilitating the refilling and ligation of the resulting single-strand DNA breaks. The removal of oxidized guanine by Ogg1 is important in preventing mutagenesis apoptosis and tumorigenesis.

Pathways

The involvement of Ogg1 can be predominantly seen in the DNA repair and oxidative stress response pathways. Within these pathways Ogg1 is closely related to other proteins such as MutYH and APE1 which work collaboratively to ensure DNA integrity. Coordinating with other repair enzymes Ogg1 therefore helps to counteract the effects of reactive oxygen species-induced damage on the genetic material.

Studies link abnormal Ogg1 activity to increased risk for cancer and neurodegenerative diseases. Mutations or deficiencies in the Ogg1 protein can result in an accumulation of 8-oxoguanine promoting genomic instability and potentially facilitating cancer development. Additionally the accumulation of oxidative DNA damage is associated with Alzheimer's disease where Ogg1 interaction with proteins like APE1 can influence disease progression by affecting the efficiency of DNA repair mechanisms.
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Specifications

Form

Liquid

Additional notes

ab98249 was purified using conventional chromatography.

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General info

Function

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

Sequence similarities

Belongs to the type-1 OGG1 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
See full target information OGG1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110788 PubMed39286502

2024

Redox-dependent condensation and cytoplasmic granulation by human ssDNA-binding protein-1 delineate roles in oxidative stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Gábor M Harami,János Pálinkás,Zoltán J Kovács,Bálint Jezsó,Krisztián Tárnok,Hajnalka Harami-Papp,József Hegedüs,Lamiya Mahmudova,Nóra Kucsma,Szilárd Tóth,Gergely Szakács,Mihály Kovács

Journal of extracellular vesicles 13:e12505 PubMed39235072

2024

The 8-oxoguanine DNA glycosylase-synaptotagmin 7 pathway increases extracellular vesicle release and promotes tumour metastasis during oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ma,Jiarong Guo,Haipeng Rao,Jingyu Xin,Xinyi Song,Rui Liu,Shan Shao,Jiajia Hou,Liyu Kong,Zhigang Hu,Lingfeng He,Feiyan Pan,Zhigang Guo

iScience 27:110459 PubMed39104412

2024

Small molecule inhibitor binds to NOD-like receptor family pyrin domain containing 3 and prevents inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Lackner,Julia Elise Cabral,Yanfei Qiu,Haitian Zhou,Lemuel Leonidas,Minh Anh Pham,Alijah Macapagal,Sophia Lin,Emy Armanus,Reginald McNulty

Bio-protocol 11:e3954 PubMed33855116

2021

Real-time Base Excision Repair Assay to Measure the Activity of the 8-oxoguanine DNA Glycosylase 1 in Isolated Mitochondria of Human Skin Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Schniertshauer,Daniel Gebhard,Jörg Bergemann

Nucleic acids research 48:6530-6546 PubMed32432680

2020

Interaction between RECQL4 and OGG1 promotes repair of oxidative base lesion 8-oxoG and is regulated by SIRT1 deacetylase.

Applications

Unspecified application

Species

Unspecified reactive species

Shunlei Duan,Xuerui Han,Mansour Akbari,Deborah L Croteau,Lene Juel Rasmussen,Vilhelm A Bohr
View all publications
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