Recombinant Human OIF protein (His tag) is a Human Full Length protein, in the 1 to 298 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M K T L Q S T L L L L L L V P L I K P A P P T Q Q D S R I I Y D Y G T D N F E E S I F S Q D Y E D K Y L D G K N I K E K E T V I I P N E K S L Q L Q K D E A I T P L P P K K E N D E M P T C L L C V C L S G S V Y C E E V D I D A V P P L P K E S A Y L Y A R F N K I K K L T A K D F A D I P N L R R L D F T G N L I E D I E D G T F S K L S L L E E L S L A E N Q L L K L P V L P P K L T L F N A K Y N K I K S R G I K A N A F K K L N N L T F L Y L D H N A L E S V P L N L P E S L R V I H L Q F N N I A S I T D D T F C K A N D T S Y I R D R I E E I R L E G N P I V L G K H P N S F I C L K R L P I G S Y F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.
Alternative names
OIF, SLRR3A, OGN, Mimecan, Osteoglycin, Osteoinductive factor
Recommended products
Recombinant Human OIF protein (His tag) is a Human Full Length protein, in the 1 to 298 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P20774-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M K T L Q S T L L L L L L V P L I K P A P P T Q Q D S R I I Y D Y G T D N F E E S I F S Q D Y E D K Y L D G K N I K E K E T V I I P N E K S L Q L Q K D E A I T P L P P K K E N D E M P T C L L C V C L S G S V Y C E E V D I D A V P P L P K E S A Y L Y A R F N K I K K L T A K D F A D I P N L R R L D F T G N L I E D I E D G T F S K L S L L E E L S L A E N Q L L K L P V L P P K L T L F N A K Y N K I K S R G I K A N A F K K L N N L T F L Y L D H N A L E S V P L N L P E S L R V I H L Q F N N I A S I T D D T F C K A N D T S Y I R D R I E E I R L E G N P I V L G K H P N S F I C L K R L P I G S Y F
- Accession
- P20774
- Protein length
- Full Length
- Predicted molecular weight
- 33.2 kDa
- Amino acids
- 1 to 298
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.
- Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.
- Post-translational modifications
O-glycosylated with a core 1 or possibly core 8 glycan.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OIF also known as Ovulation Inducing Factor has a mass of about 14 kDa. It plays an important role in inducing ovulation in certain mammalian species. OIF is expressed in seminal plasma where it serves as a signaling molecule influencing the reproductive cycle. Researchers recognize the impact of OIF on the ovarian follicle where it initiates a cascade of events that lead to ovulation. Its discovery is significant for reproductive biology as it highlights the influence of male factors on female reproductive physiology.
- Biological function summary
Ovulation Inducing Factor acts as a signaling molecule that interacts with the female reproductive system to trigger ovulation. It functions as part of a larger biochemical complex that affects the hypothalamus and pituitary gland axis stimulating the secretion of hormones necessary for the release of the egg. The exact interactions and pathways remain an active area of research but the ability of OIF to prompt ovulation positions it as a unique factor among reproductive proteins.
- Pathways
There is evidence that Ovulation Inducing Factor is involved in the neuroendocrine pathway for ovulation. OIF interacts with the hypothalamus-pituitary-gonadal axis to initiate ovulation. The presence of other proteins like GnRH and its release is closely tied to the activity of OIF in stimulating the necessary hormonal environment. Furthermore the interplay between OIF and luteinizing hormone (LH) is also explored showing how these elements integrate into the broader signaling cascade essential for ovulation.
- Associated diseases and disorders
Researchers have associated Ovulation Inducing Factor with fertility issues. Disruptions or alterations in the pathway involving OIF can lead to ovulatory disorders impacting fertility in both females and indirectly males. Studies explore its relationship with proteins like FSH and LH which are critical in ovulatory processes. Understanding the role of OIF may advance treatment options for certain infertility problems offering insights into novel therapeutic strategies.
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SDS-PAGE - Recombinant Human OIF protein (His tag) (ab276546)
SDS-PAGE analysis of ab276546
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