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AB136379

Recombinant Human OPRT protein (denatured)

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Recombinant Human OPRT protein (denatured) is a Human Full Length protein, in the 1 to 480 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

OK/SW-cl.21, UMPS, Uridine 5'-monophosphate synthase, UMP synthase

1 Images
SDS-PAGE - Recombinant Human OPRT protein (denatured) (AB136379)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human OPRT protein (denatured) (AB136379)

15% SDS-PAGE analysis of ab136379 (3 µg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P11172

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 12.01% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as UMPS
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIVSRPRLLSQVADILFQTAQNAGISFDTVCGVPYTALPLATVICSTNQIPMLIRRKETKDYGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQKEGLKVTDAIVLLDREQGGKDKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRFIQENVFVAANHNGSPLSIKEAPKELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPSICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIASWADLVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEEHSEFVVGFISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVGRGIISAADRLEAAEMYRKAAWEAYLSRLGV","proteinLength":"Full Length","predictedMolecularWeight":"54.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":480,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P11172","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orotate phosphoribosyltransferase often abbreviated as OPRT or known by its enzyme commission number EC 2.4.2.10 plays a significant role in pyrimidine biosynthesis. This enzyme with a molecular weight of around 23 kDa catalyzes the conversion of orotate and 5-phosphoribosyl-1-pyrophosphate (PRPP) into orotidine-5'-monophosphate (OMP). OPRT is expressed in various tissues with notably higher levels in rapidly proliferating cells due to their increased demand for nucleotides.
Biological function summary

Orotate phosphoribosyltransferase contributes to the de novo synthesis of pyrimidine nucleotides which are essential components of DNA and RNA. OPRT operates as part of the UMP biosynthesis via the de novo pathway forming a complex with orotidine-5'-phosphate decarboxylase in some organisms. This linkage enhances efficiency in pyrimidine nucleotide production reflecting its central role in cell growth and division.

Pathways

The protein integrates into the pyrimidine metabolism pathway. It interacts closely with orotate phosphoribosyltransferase and orotidine-5'-phosphate decarboxylase forming dual-function enzymes that streamline UMP synthesis. OPRT’s activity ties it to key metabolic cycles including nucleotide salvage pathways thereby establishing critical links across metabolic networks essential for nucleic acid production.

Dysfunction in Orotate phosphoribosyltransferase can lead to hereditary orotic aciduria a rare autosomal recessive disorder characterized by megaloblastic anemia and excessive orotate excretion. Changes in OPRT activity also impact pyrimidine antimetabolite drug efficacy such as 5-fluorouracil demonstrating its significance in cancer treatment. Additionally OPRT's interactions with dihydroorotate dehydrogenase can be important in understanding metabolic disturbances related to pyrimidine disorders.
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Specifications

Form

Liquid

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General info

Function

Bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis, orotate phosphoribosyltransferase (OPRT), which converts orotate to orotidine-5'-monophosphate (OMP), and orotidine-5'-monophosphate decarboxylase (ODC), the terminal enzymatic reaction that decarboxylates OMP to uridine monophosphate (UMP).

Sequence similarities

In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.. In the C-terminal section; belongs to the OMP decarboxylase family.

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Product protocols

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Target data

Bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis, orotate phosphoribosyltransferase (OPRT), which converts orotate to orotidine-5'-monophosphate (OMP), and orotidine-5'-monophosphate decarboxylase (ODC), the terminal enzymatic reaction that decarboxylates OMP to uridine monophosphate (UMP).
See full target information UMPS
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Complementary Products

Primary Antibodies

AB155763

Anti-OPRT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPRT antibody (AB155763)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB111101

Anti-F4/80 antibody [SP115]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (AB111101)

  • 4
  • 20
Primary Antibodies

AB205921

Anti-PD-L1 antibody [28-8]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (AB205921)

  • 4
  • 15

Product promise

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