Recombinant Human OPRT protein (His tag) is a Human Full Length protein, in the 1 to 480 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A V A R A A L G P L V T G L Y D V Q A F K F G D F V L K S G L S S P I Y I D L R G I V S R P R L L S Q V A D I L F Q T A Q N A G I S F D T V C G V P Y T A L P L A T V I C S T N Q I P M L I R R K E T K D Y G T K R L V E G T I N P G E T C L I I E D V V T S G S S V L E T V E V L Q K E G L K V T D A I V L L D R E Q G G K D K L Q A H G I R L H S V C T L S K M L E I L E Q Q K K V D A E T V G R V K R F I Q E N V F V A A N H N G S P L S I K E A P K E L S F G A R A E L P R I H P V A S K L L R L M Q K K E T N L C L S A D V S L A R E L L Q L A D A L G P S I C M L K T H V D I L N D F T L D V M K E L I T L A K C H E F L I F E D R K F A D I G N T V K K Q Y E G G I F K I A S W A D L V N A H V V P G S G V V K G L Q E V G L P L H R G C L L I A E M S S T G S L A T G D Y T R A A V R M A E E H S E F V V G F I S G S R V S M K P E F L H L T P G V Q L E A G G D N L G Q Q Y N S P Q E V I G K R G S D I I I V G R G I I S A A D R L E A A E M Y R K A A W E A Y L S R L G V H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis, orotate phosphoribosyltransferase (OPRT), which converts orotate to orotidine-5'-monophosphate (OMP), and orotidine-5'-monophosphate decarboxylase (ODC), the terminal enzymatic reaction that decarboxylates OMP to uridine monophosphate (UMP).
Alternative names
OK/SW-cl.21, UMPS, Uridine 5'-monophosphate synthase, UMP synthase
Recommended products
Recombinant Human OPRT protein (His tag) is a Human Full Length protein, in the 1 to 480 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P11172-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS
Sequence info
Amino acid sequence
- M A V A R A A L G P L V T G L Y D V Q A F K F G D F V L K S G L S S P I Y I D L R G I V S R P R L L S Q V A D I L F Q T A Q N A G I S F D T V C G V P Y T A L P L A T V I C S T N Q I P M L I R R K E T K D Y G T K R L V E G T I N P G E T C L I I E D V V T S G S S V L E T V E V L Q K E G L K V T D A I V L L D R E Q G G K D K L Q A H G I R L H S V C T L S K M L E I L E Q Q K K V D A E T V G R V K R F I Q E N V F V A A N H N G S P L S I K E A P K E L S F G A R A E L P R I H P V A S K L L R L M Q K K E T N L C L S A D V S L A R E L L Q L A D A L G P S I C M L K T H V D I L N D F T L D V M K E L I T L A K C H E F L I F E D R K F A D I G N T V K K Q Y E G G I F K I A S W A D L V N A H V V P G S G V V K G L Q E V G L P L H R G C L L I A E M S S T G S L A T G D Y T R A A V R M A E E H S E F V V G F I S G S R V S M K P E F L H L T P G V Q L E A G G D N L G Q Q Y N S P Q E V I G K R G S D I I I V G R G I I S A A D R L E A A E M Y R K A A W E A Y L S R L G V H H H H H H
- Accession
- P11172
- Protein length
- Full Length
- Predicted molecular weight
- 53 kDa
- Amino acids
- 1 to 480
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis, orotate phosphoribosyltransferase (OPRT), which converts orotate to orotidine-5'-monophosphate (OMP), and orotidine-5'-monophosphate decarboxylase (ODC), the terminal enzymatic reaction that decarboxylates OMP to uridine monophosphate (UMP).
- Sequence similarities
In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as UMPS
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Orotate phosphoribosyltransferase often abbreviated as OPRT or known by its enzyme commission number EC 2.4.2.10 plays a significant role in pyrimidine biosynthesis. This enzyme with a molecular weight of around 23 kDa catalyzes the conversion of orotate and 5-phosphoribosyl-1-pyrophosphate (PRPP) into orotidine-5'-monophosphate (OMP). OPRT is expressed in various tissues with notably higher levels in rapidly proliferating cells due to their increased demand for nucleotides.
- Biological function summary
Orotate phosphoribosyltransferase contributes to the de novo synthesis of pyrimidine nucleotides which are essential components of DNA and RNA. OPRT operates as part of the UMP biosynthesis via the de novo pathway forming a complex with orotidine-5'-phosphate decarboxylase in some organisms. This linkage enhances efficiency in pyrimidine nucleotide production reflecting its central role in cell growth and division.
- Pathways
The protein integrates into the pyrimidine metabolism pathway. It interacts closely with orotate phosphoribosyltransferase and orotidine-5'-phosphate decarboxylase forming dual-function enzymes that streamline UMP synthesis. OPRT’s activity ties it to key metabolic cycles including nucleotide salvage pathways thereby establishing critical links across metabolic networks essential for nucleic acid production.
- Associated diseases and disorders
Dysfunction in Orotate phosphoribosyltransferase can lead to hereditary orotic aciduria a rare autosomal recessive disorder characterized by megaloblastic anemia and excessive orotate excretion. Changes in OPRT activity also impact pyrimidine antimetabolite drug efficacy such as 5-fluorouracil demonstrating its significance in cancer treatment. Additionally OPRT's interactions with dihydroorotate dehydrogenase can be important in understanding metabolic disturbances related to pyrimidine disorders.
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1 product image
SDS-PAGE - Recombinant Human OPRT protein (His tag) (ab226883)
15% SDS-PAGE analysis of 3 μg ab226883.
MW: 50-70 kDa (SDS-PAGE under reducing conditions).
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