Recombinant Human Opticin protein (denatured) is a Human Full Length protein, in the 20 to 332 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S A S L P R K E R K R R E E Q M P R E G D S F E V L P L R N D V L N P D N Y G E V I D L S N Y E E L T D Y G D Q L P E V K V T S L A P A T S I S P A K S T T A P G T P S S N P T M T R P T T A G L L L S S Q P N H G L P T C L V C V C L G S S V Y C D D I D L E D I P P L P R R T A Y L Y A R F N R I S R I R A E D F K G L T K L K R I D L S N N L I S S I D N D A F R L L H A L Q D L I L P E N Q L E A L P V L P S G I E F L D V R L N R L Q S S G I Q P A A F R A M E K L Q F L Y L S D N L L D S I P G P L P L S L R S V H L Q N N L I E T M Q R D V F C D P E E H K H T R R Q L E D I R L D G N P I N L S L F P S A Y F C L P R L P I G R F T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibits angiogenesis in the vitreous humor of the eye, and therefore represses neovascularization (By similarity). Binds collagen fibrils (By similarity). May be involved in collagen fiber organization via regulation of other members of the small leucine-rich repeat proteoglycan superfamily (By similarity).
Alternative names
OPT, OPTC, Opticin, Oculoglycan
Recommended products
Recombinant Human Opticin protein (denatured) is a Human Full Length protein, in the 20 to 332 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9UBM4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S A S L P R K E R K R R E E Q M P R E G D S F E V L P L R N D V L N P D N Y G E V I D L S N Y E E L T D Y G D Q L P E V K V T S L A P A T S I S P A K S T T A P G T P S S N P T M T R P T T A G L L L S S Q P N H G L P T C L V C V C L G S S V Y C D D I D L E D I P P L P R R T A Y L Y A R F N R I S R I R A E D F K G L T K L K R I D L S N N L I S S I D N D A F R L L H A L Q D L I L P E N Q L E A L P V L P S G I E F L D V R L N R L Q S S G I Q P A A F R A M E K L Q F L Y L S D N L L D S I P G P L P L S L R S V H L Q N N L I E T M Q R D V F C D P E E H K H T R R Q L E D I R L D G N P I N L S L F P S A Y F C L P R L P I G R F T
- Accession
- Q9UBM4
- Protein length
- Full Length
- Predicted molecular weight
- 37.6 kDa
- Amino acids
- 20 to 332
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab202160 was purified by conventional chromatography techniques.
General info
- Function
Inhibits angiogenesis in the vitreous humor of the eye, and therefore represses neovascularization (By similarity). Binds collagen fibrils (By similarity). May be involved in collagen fiber organization via regulation of other members of the small leucine-rich repeat proteoglycan superfamily (By similarity).
- Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.
- Post-translational modifications
O-glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Opticin also known as OPN or Myocilin-Associated Sugar TATA-associated peptide-like protein is a small leucine-rich repeat protein with a molecular mass of approximately 37 kDa. It localizes mainly in the vitreous body of the eye and is expressed in several tissues including the retina and ciliary body. This protein has a role in collagen fibrillogenesis regulation by binding to collagen which prevents its abnormal aggregation ensuring proper structure and function of the extracellular matrix.
- Biological function summary
Opticin plays a role in maintaining eye health by modulating cell adhesion and inhibiting angiogenesis. It contributes to preventing unwanted blood vessel growth in ocular tissues which can lead to complications. Opticin is not part of a larger protein complex but it interacts dynamically with the extracellular matrix and other matrix-associated proteins that include vitronectin and thrombospondin.
- Pathways
Opticin participates in pathways regulating tissue remodeling and vascular development. Within the extracellular matrix organization pathway opticin works with proteins such as fibronectin and decorin to maintain tissue integrity. Additionally opticin contributes to the angiogenesis inhibition pathway where it helps to regulate new blood vessel formation alongside thrombospondin-1 acting as an inhibitor of angiogenic processes.
- Associated diseases and disorders
Opticin has connections to ocular diseases and inflammatory conditions. It is linked to age-related macular degeneration (AMD) a disorder where dysfunctional vascular growth occurs and opticin's role in Vascular Endothelial Growth Factor (VEGF) inhibition offers therapeutic insights. Additionally its interaction with complement factor H suggests a connection to inflammatory processes where opticin could help regulate immune responses. Understanding opticin’s functions better may help develop targeted treatments for these conditions.
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1 product image
SDS-PAGE - Recombinant Human Opticin protein (denatured) (ab202160)
15% SDS-PAGE analysis of ab202160 (3μg).
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