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AB153049

Recombinant Human Optineurin protein (GST tag N-Terminus)

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Recombinant Human Optineurin protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 571 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

FIP2, GLC1E, HIP7, HYPL, NRP, OPTN, Optineurin, E3-14.7K-interacting protein, Huntingtin yeast partner L, Huntingtin-interacting protein 7, Huntingtin-interacting protein L, NEMO-related protein, Optic neuropathy-inducing protein, Transcription factor IIIA-interacting protein, FIP-2, HIP-7, TFIIIA-IntP

1 Images
SDS-PAGE - Recombinant Human Optineurin protein (GST tag N-Terminus) (AB153049)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Optineurin protein (GST tag N-Terminus) (AB153049)

ab153049 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q96CV9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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  • 0
Primary Antibodies

AB213556

Anti-Optineurin antibody [EPR20654]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Optineurin antibody [EPR20654] (AB213556)

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Primary Antibodies

AB229489

Anti-NEK1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NEK1 antibody (AB229489)

  • 0
  • 0
Primary Antibodies

AB169871

Anti-SNAP25 (phospho S187) antibody

Western blot - Anti-SNAP25 (phospho S187) antibody (AB169871)

  • 0
  • 0
Primary Antibodies

AB238144

Anti-WWOX antibody [EPR23109-138]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-WWOX antibody [EPR23109-138] (AB238144)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":571,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96CV9","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Optineurin also known as OPTN is a multifunctional protein of approximately 67 kDa. It is expressed in various tissues including the retina where it plays a role in maintaining cellular functions. Optineurin interacts with several binding partners to execute its roles within the cell. It is involved in intracellular trafficking signaling and inflammation processes. As an essential component of the cellular machinery optineurin aids in proper cellular communication and responses.
Biological function summary

Optineurin influences different cellular processes notably autophagy and regulation of inflammation. It participates in forming a complex with other proteins to facilitate cargo transport and degradation via the autophagy-lysosome pathway. This function is important for removing damaged proteins and organelles ensuring cellular homeostasis. Inflammation regulation occurs through optineurin's ability to inhibit certain signaling pathways therefore modulating immune responses and preventing excessive inflammation.

Pathways

Optineurin plays a role in the NF-kB signaling pathway and the autophagic process. In NF-kB signaling it acts as a negative regulator by degrading specific signaling molecules. This regulation is necessary to prevent chronic inflammation and maintain immune balance. Within autophagy optineurin collaborates with other autophagy-related proteins like LC3 to facilitate the sequestration and clearance of cellular components preventing accumulation of damaged cellular materials.

Optineurin connects to conditions like glaucoma and amyotrophic lateral sclerosis (ALS). Mutations in the optineurin gene link to glaucoma as they can impair autophagy and lead to optic nerve damage. In the context of ALS optineurin mutations disrupt normal cellular functions contributing to motor neuron degeneration. The protein TDP-43 is often associated with ALS and interacts with optineurin in pathological processes affecting disease progression.
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Specifications

Form

Liquid

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General info

Function

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8 (PubMed : 27534431). Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation (PubMed : 27534431). Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation (PubMed : 27538435). In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta/IFNB1. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and huntingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as that of transferrin receptor (TFRC/TfR); regulates Rab8 recruitment to tubules emanating from the endocytic recycling compartment (PubMed : 22854040). Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52.. (Microbial infection) May constitute a cellular target for various viruses, such as adenovirus E3 14.7 or Bluetongue virus, to inhibit innate immune response (PubMed : 27538435, PubMed : 9488477). During RNA virus infection, such as that of Sendai virus, negatively regulates the induction of IFNB1 (PubMed : 20174559).

Post-translational modifications

Phosphorylated by TBK1, leading to restrict bacterial proliferation in case of infection. Phosphorylation is induced by phorbol esters and decreases its half-time.

Subcellular localisation

Recycling endosome

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Product protocols

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Target data

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8 (PubMed : 27534431). Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation (PubMed : 27534431). Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation (PubMed : 27538435). In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta/IFNB1. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and huntingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as that of transferrin receptor (TFRC/TfR); regulates Rab8 recruitment to tubules emanating from the endocytic recycling compartment (PubMed : 22854040). Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52.. (Microbial infection) May constitute a cellular target for various viruses, such as adenovirus E3 14.7 or Bluetongue virus, to inhibit innate immune response (PubMed : 27538435, PubMed : 9488477). During RNA virus infection, such as that of Sendai virus, negatively regulates the induction of IFNB1 (PubMed : 20174559).
See full target information OPTN
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