Recombinant Human ornithine aminotransferase protein is a Human Full Length protein, in the 33 to 439 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M T V Q G P P T S D D I F E R E Y K Y G A H N Y H P L P V A L E R G K G I Y L W D V E G R K Y F D F L S S Y S A V N Q G H C H P K I V N A L K S Q V D K L T L T S R A F Y N N V L G E Y E E Y I T K L F N Y H K V L P M N T G V E A G E T A C K L A R K W G Y T V K G I Q K Y K A K I V F A A G N F W G R T L S A I S S S T D P T S Y D G F G P F M P G F D I I P Y N D L P A L E R A L Q D P N V A A F M V E P I Q G E A G V V V P D P G Y L M G V R E L C T R H Q V L F I A D E I Q T G L A R T G R W L A V D Y E N V R P D I V L L G K A L S G G L Y P V S A V L C D D D I M L T I K P G E H G S T Y G G N P L G C R V A I A A L E V L E E E N L A E N A D K L G I I L R N E L M K L P S D V V T A V R G K G L L N A I V I K E T K D W D A W K V C L R L R D N G L L A K P T H G D I I R F A P P L V I K E D E L R E S I E I I N K T I L S F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the reversible interconversion of L-ornithine and 2-oxoglutarate to L-glutamate semialdehyde and L-glutamate.
Alternative names
Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase, OAT
Recommended products
Recombinant Human ornithine aminotransferase protein is a Human Full Length protein, in the 33 to 439 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P04181-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M T V Q G P P T S D D I F E R E Y K Y G A H N Y H P L P V A L E R G K G I Y L W D V E G R K Y F D F L S S Y S A V N Q G H C H P K I V N A L K S Q V D K L T L T S R A F Y N N V L G E Y E E Y I T K L F N Y H K V L P M N T G V E A G E T A C K L A R K W G Y T V K G I Q K Y K A K I V F A A G N F W G R T L S A I S S S T D P T S Y D G F G P F M P G F D I I P Y N D L P A L E R A L Q D P N V A A F M V E P I Q G E A G V V V P D P G Y L M G V R E L C T R H Q V L F I A D E I Q T G L A R T G R W L A V D Y E N V R P D I V L L G K A L S G G L Y P V S A V L C D D D I M L T I K P G E H G S T Y G G N P L G C R V A I A A L E V L E E E N L A E N A D K L G I I L R N E L M K L P S D V V T A V R G K G L L N A I V I K E T K D W D A W K V C L R L R D N G L L A K P T H G D I I R F A P P L V I K E D E L R E S I E I I N K T I L S F
- Accession
- P04181
- Protein length
- Full Length
- Amino acids
- 33 to 439
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab92933 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the reversible interconversion of L-ornithine and 2-oxoglutarate to L-glutamate semialdehyde and L-glutamate.
- Sequence similarities
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ornithine aminotransferase also known as ornithine transaminase is an enzyme that facilitates the conversion of ornithine and α-ketoglutarate to glutamate semialdehyde and glutamate. This enzyme weighs around 49 kDa. It shows expression mainly in the liver kidney small intestine and retina. The enzyme works inside cellular mitochondria and assists by catalyzing amino group transfer reactions. It is one member of the transaminase family.
- Biological function summary
This enzyme plays a role in the urea cycle and amino acid metabolism. It helps in the conversion of excess nitrogen into less toxic forms that the body can excrete. As a part of an important metabolic network it influences the synthesis and degradation of important biological molecules. It operates independently and does not form known protein complexes for its function.
- Pathways
Ornithine aminotransferase is important in the ornithine and glutamate metabolism pathways. Within the urea cycle it cooperates with enzymes such as arginase and carbamoyl phosphate synthetase to regulate nitrogen balance in cells. Additionally it engages in the proline-glutamate pathway which involves proteins like proline dehydrogenase analzying the reversible conversion between proline and glutamate.
- Associated diseases and disorders
Ornithine aminotransferase associates with gyrate atrophy a rare genetic condition that affects the eye and leads to vision loss. Deficiencies in this enzyme can result in abnormal accumulations of ornithine in the blood. Some connections also exist with hyperammonemia where impaired nitrogen excretion causes elevated ammonia levels. In these conditions the enzyme's defective activity links to mutations or irregular interactions with proteins like glutamate dehydrogenase affecting metabolic balance.
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1 product image
SDS-PAGE - Recombinant Human ornithine aminotransferase protein (ab92933)
ab92933, staining ornithine aminotransferase protein by 15% SDS-PAGE (3 μg).
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