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AB123452

Recombinant Human Ornithine Carbamoyltransferase/OTC protein

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Recombinant Human Ornithine Carbamoyltransferase/OTC protein is a Human Full Length protein in the 1 to 354 aa range with >80% purity and suitable for western blot, SDS-PAGE and ELISA. The predicted molecular weight of ab123452 protein is 40.7 kDa.

- Save time and ensure accurate results - use our recombinant OTC protein as a control

View Alternative Names

OTCase, OTC

2 Images
Western blot - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (AB123452)
  • WB

Unknown

Western blot - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (AB123452)

All lanes:

Anti-Ornithine Carbamoyltransferase antibody (<a href='/en-us/products/unavailable/ornithine-carbamoyltransferaseotc-antibody-ab86665'>ab86665</a>) at 0.5 µg/mL

All lanes:

Western blot - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452) at 0.01 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution

true

Exposure time: 5min

SDS-PAGE - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (AB123452)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (AB123452)

10-20% acrylamide separation gel showing ab123452 at approximately 40kDa (1μg).

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

P00480

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.15% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our recombinant human Ornithine Carbamoyltransferase/OTC protein ab123452 as a positive control in western blot and SDS-PAGE.

Analyze your OTC ELISA data using the ab123452 protein to generate and plot a standard curve.


Check out of western blot protocol for more information here

Check out our protein gel staining guide for SDS-PAGE here

Check out our ELISA protocol for more information here.


Product was previously marketed under the MitoSciences sub-brand. Previously labelled as Ornithine Carbamoyltransferase.
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Sequence info

[{"sequence":"MLFNLRILLNNAAFRNGHNFMVRNFRCGQPLQNKVQLKGRDLLTLKNFTGEEIKYMLWLSADLKFRIKQKGEYLPLLQGKSLGMIFEKRSTRTRLSTETGFALLGGHPCFLTTQDIHLGVNESLTDTARVLSSMADAVLARVYKQSDLDTLAKEASIPIINGLSDLYHPIQILADYLTLQEHYSSLKGLTLSWIGDGNNILHSIMMSAAKFGMHLQAATPKGYEPDASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMGQEEEKKKRLQAFQGYQVTMKTAKVAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFPEAENRKWTIMAVMVSLLTDYSPQLQKPKFHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"40.76 kDa","actualMolecularWeight":null,"aminoAcidEnd":354,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00480","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ornithine Carbamoyltransferase (OTC) also known as ornithine aminotransferase is an enzyme critical in the urea cycle. The OTC protein facilitates the conversion of ornithine and carbamoyl phosphate into citrulline a process essential for nitrogen excretion. The human OTC protein has a mass of approximately 36 kilodaltons and is primarily expressed in the liver mitochondria. Protein assays and procurement efforts often focus on obtaining accurate measurements and ensuring high-purity OTC peptides enabling proper functional studies.
Biological function summary

Ornithine carbamoyltransferase plays a significant role in detoxifying ammonia by forming citrulline an important urea cycle intermediate. The protein functions as a homotrimer meaning three identical subunits form an active enzyme complex. This multimeric state facilitates efficient substrate processing allowing effective handling of nitrogen waste. The integral role of OTC in the urea cycle highlights its importance in maintaining systemic nitrogen balance.

Pathways

OTC is a central component of the urea cycle and influences nitrogen metabolism pathways. These pathways include the conversion of ammonia to urea an important detoxifying process for safe excretion by the kidneys. OTC closely interacts with enzymes such as argininosuccinate synthetase highlighting the coordinated action required to advance urea formation. Understanding OTC's participation within these pathways helps identify its regulatory points and the potential impact of genetic variations.

Mutations in the OTC gene can lead to ornithine transcarbamylase deficiency a disorder affecting ammonia detoxification. This X-linked genetic condition disrupts normal nitrogen metabolism causing ammonia accumulation particularly harmful to the central nervous system. OTC deficiency shares a functional relationship with other urea cycle disorders such as citrullinemia due to its contribution to the same biochemical pathway. Researchers focus on identifying OTC protein mutations to improve diagnosis and treatment of these conditions.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline (PubMed : 2556444, PubMed : 6372096, PubMed : 8112735). The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion (PubMed : 2556444).

Sequence similarities

Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.

Post-translational modifications

Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline (PubMed : 2556444, PubMed : 6372096, PubMed : 8112735). The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion (PubMed : 2556444).
See full target information Ornithine transcarbamylase, mitochondrial
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Product promise

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