Recombinant Human ORP150 protein (His tag)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORP150 also known as Heat Shock Protein 12A (HSPA12A) is a molecular chaperone with an approximate mass of 150 kDa. This protein plays a critical role in protein folding and preventing misfolded proteins in the endoplasmic reticulum (ER). ORP150 is mainly expressed in cells that face challenging conditions like those in the brain heart and tumors. Its expression indeed increases under stress conditions that cause protein unfolding such as hypoxia or glucose deprivation.

Biological function summary

ORP150 functions to aid cellular adaptation during stress by maintaining protein homeostasis within the ER. It is recognized as part of the heat shock protein family which is known for its involvement in cellular protection mechanisms. By stabilizing polypeptides and proteins ORP150 contributes significantly to cellular survival under various stress conditions. Moreover its expression under hypoxic conditions suggests ORP150's integral role in such stressful environments helping to ameliorate oxidative damage to cells.

Pathways

ORP150 integrates into the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways. These pathways help in the maintenance of protein quality control within the ER. Within the UPR ORP150 collaborates with proteins like GRP78 another chaperone to manage misfolded proteins and ensure proper maturation of nascent chains. Furthermore ORP150 participates in ERAD where it assists in targeting misfolded proteins for degradation therefore managing cellular stress and maintaining cellular health.

ORP150 has associations with neurodegenerative diseases like Alzheimer's disease and cardiovascular disorders. In conditions such as Alzheimer's the accumulation of misfolded proteins can exacerbate the disease state where ORP150 potentially interacts with amyloid precursor protein (APP) to regulate its folding and processing. Similarly in cardiovascular diseases ORP150 may influence the function of related proteins like GRP78 as both play roles in protecting cardiac cells from ischemic damage. The consistent association of ORP150 expression with stressed cellular environments suggests its importance in the pathology and management of these conditions.