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AB152583

Recombinant Human OSBP1 protein (GST tag N-Terminus)

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Recombinant Human OSBP1 protein (GST tag N-Terminus) is a Human Fragment protein, in the 324 to 407 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

OSBP1, OSBP, Oxysterol-binding protein 1

1 Images
SDS-PAGE - Recombinant Human OSBP1 protein (GST tag N-Terminus) (AB152583)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human OSBP1 protein (GST tag N-Terminus) (AB152583)

12.5% SDS-PAGE analysis of ab152583 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P22059

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB192995

Anti-OSBP1 antibody

Western blot - Anti-OSBP1 antibody (AB192995)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB165052

Recombinant Human OSBPL9 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human OSBPL9 protein (GST tag N-Terminus) (AB165052)

  • 0
  • 0
Primary Antibodies

AB70722

Anti-SEC16A/SEC16 antibody

Immunoprecipitation - Anti-SEC16A/SEC16 antibody (AB70722)

  • 4
  • 6
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"GATVLPANTPGNVGSGKDQCCSGKGDMSDEDDENEFFDAPEIITMPENLGHKRTGSNISGASSDISLDEQYKHQLEETKKEKRT","proteinLength":"Fragment","predictedMolecularWeight":"34.98 kDa","actualMolecularWeight":null,"aminoAcidEnd":407,"aminoAcidStart":324,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P22059","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oxysterol-binding protein 1 (OSBP1) also known as OSBP is a protein with a molecular mass of approximately 97 kilodaltons. It functions as an intracellular lipid transporter that binds oxysterols which are oxygenated derivatives of cholesterol. OSBP1 is involved in lipid exchange between the endoplasmic reticulum (ER) and the Golgi apparatus. It is expressed in various tissues with high levels observed in the liver brain and adipose tissue. OSBP1 facilitates the transport of sterols and phosphatidylinositol 4-phosphate (PI4P) influencing lipid metabolism and membrane formation.
Biological function summary

OSBP1 plays a significant role in maintaining cellular lipid homeostasis and is a part of the larger family of oxysterol-binding proteins. It participates in intracellular lipid transport and signaling aiding in the regulation of cholesterol metabolism. OSBP1 coordinates lipid exchange processes between the ER and other cellular compartments impacting membrane composition and function. Its activity is important for the generation of certain lipid domains contributing to cellular signaling pathways and membrane trafficking.

Pathways

OSBP1 interacts within complex lipid signaling networks and is critically involved in the regulation of the phosphoinositide and cholesterol pathways. It collaborates with proteins such as VAP-A at membrane contact sites to exchange lipids like cholesterol and PI4P. This interaction is key to maintaining lipid gradients essential for multiple cellular functions including signaling and vesicle trafficking. Additionally OSBP1 modulates the activity of downstream phosphatidylinositol 4-kinases affecting phosphoinositide metabolism.

OSBP1's dysregulation associates with abnormalities in lipid metabolism and related diseases. Such alterations can contribute to metabolic disorders like atherosclerosis due to its involvement in cholesterol transport and distribution. Furthermore OSBP1's function is linked to the pathogenesis of Niemann-Pick disease type C where cholesterol accumulation occurs. In these contexts OSBP1 operates in concert with other proteins such as Niemann-Pick C1 (NPC1) that participate in cholesterol trafficking and metabolism.
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Specifications

Form

Liquid

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General info

Function

Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum : specifically exchanges sterol (cholesterol) with phosphatidylinositol 4-phosphate (PI4P, 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)), delivering sterol to the Golgi in exchange for PI4P, which is subsequently degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum (PubMed : 24209621, PubMed : 28978670). Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol (PubMed : 15746430, PubMed : 17428193). Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly (PubMed : 15746430). Regulates cholesterol efflux by decreasing the stability of the relatively short lived ABCA1 protein (an important point of control for cholesterol efflux activity) (PubMed : 18450749).

Sequence similarities

Belongs to the OSBP family.

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Product protocols

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Target data

Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum : specifically exchanges sterol (cholesterol) with phosphatidylinositol 4-phosphate (PI4P, 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)), delivering sterol to the Golgi in exchange for PI4P, which is subsequently degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum (PubMed : 24209621, PubMed : 28978670). Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol (PubMed : 15746430, PubMed : 17428193). Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly (PubMed : 15746430). Regulates cholesterol efflux by decreasing the stability of the relatively short lived ABCA1 protein (an important point of control for cholesterol efflux activity) (PubMed : 18450749).
See full target information OSBP
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