Recombinant Human OSBP1 protein is a Human Fragment protein, in the 324 to 407 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
G A T V L P A N T P G N V G S G K D Q C C S G K G D M S D E D D E N E F F D A P E I I T M P E N L G H K R T G S N I S G A S S D I S L D E Q Y K H Q L E E T K K E K R T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum (PubMed:24209621). Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol (PubMed:15746430, PubMed:17428193). Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly (PubMed:15746430). Regulates cholesterol efflux by decreasing ABCA1 stability (PubMed:18450749).
Alternative names
OSBP1, OSBP, Oxysterol-binding protein 1
Recommended products
Recombinant Human OSBP1 protein is a Human Fragment protein, in the 324 to 407 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P22059-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- G A T V L P A N T P G N V G S G K D Q C C S G K G D M S D E D D E N E F F D A P E I I T M P E N L G H K R T G S N I S G A S S D I S L D E Q Y K H Q L E E T K K E K R T
- Accession
- P22059
- Protein length
- Fragment
- Predicted molecular weight
- 34.98 kDa
- Amino acids
- 324 to 407
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum (PubMed:24209621). Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol (PubMed:15746430, PubMed:17428193). Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly (PubMed:15746430). Regulates cholesterol efflux by decreasing ABCA1 stability (PubMed:18450749).
- Sequence similarities
Belongs to the OSBP family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oxysterol-binding protein 1 (OSBP1) also known as OSBP is a protein with a molecular mass of approximately 97 kilodaltons. It functions as an intracellular lipid transporter that binds oxysterols which are oxygenated derivatives of cholesterol. OSBP1 is involved in lipid exchange between the endoplasmic reticulum (ER) and the Golgi apparatus. It is expressed in various tissues with high levels observed in the liver brain and adipose tissue. OSBP1 facilitates the transport of sterols and phosphatidylinositol 4-phosphate (PI4P) influencing lipid metabolism and membrane formation.
- Biological function summary
OSBP1 plays a significant role in maintaining cellular lipid homeostasis and is a part of the larger family of oxysterol-binding proteins. It participates in intracellular lipid transport and signaling aiding in the regulation of cholesterol metabolism. OSBP1 coordinates lipid exchange processes between the ER and other cellular compartments impacting membrane composition and function. Its activity is important for the generation of certain lipid domains contributing to cellular signaling pathways and membrane trafficking.
- Pathways
OSBP1 interacts within complex lipid signaling networks and is critically involved in the regulation of the phosphoinositide and cholesterol pathways. It collaborates with proteins such as VAP-A at membrane contact sites to exchange lipids like cholesterol and PI4P. This interaction is key to maintaining lipid gradients essential for multiple cellular functions including signaling and vesicle trafficking. Additionally OSBP1 modulates the activity of downstream phosphatidylinositol 4-kinases affecting phosphoinositide metabolism.
- Associated diseases and disorders
OSBP1's dysregulation associates with abnormalities in lipid metabolism and related diseases. Such alterations can contribute to metabolic disorders like atherosclerosis due to its involvement in cholesterol transport and distribution. Furthermore OSBP1's function is linked to the pathogenesis of Niemann-Pick disease type C where cholesterol accumulation occurs. In these contexts OSBP1 operates in concert with other proteins such as Niemann-Pick C1 (NPC1) that participate in cholesterol trafficking and metabolism.
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1 product image
SDS-PAGE - Recombinant Human OSBP1 protein (ab152583)
12.5% SDS-PAGE analysis of ab152583 stained with Coomassie Blue.
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