Recombinant Human OSGEP protein (denatured) is a Human Full Length protein, in the 1 to 335 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M P A V L G F E G S A N K I G V G V V R D G K V L A N P R R T Y V T P P G T G F L P G D T A R H H R A V I L D L L Q E A L T E S G L T S Q D I D C I A Y T K G P G M G A P L V S V A V V A R T V A Q L W N K P L V G V N H C I G H I E M G R L I T G A T S P T V L Y V S G G N T Q V I A Y S E H R Y R I F G E T I D I A V G N C L D R F A R V L K I S N D P S P G Y N I E Q M A K R G K K L V E L P Y T V K G M D V S F S G I L S F I E D V A H R M L A T G E C T P E D L C F S L Q E T V F A M L V E I T E R A M A H C G S Q E A L I V G G V G C N V R L Q E M M A T M C Q E R G A R L F A T D E R F C I D N G A M I A Q A G W E M F R A G H R T P L S D S G V T Q R Y R T D E V E V T W R D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.
Alternative names
GCPL1, OSGEP, tRNA N6-adenosine threonylcarbamoyltransferase, N6-L-threonylcarbamoyladenine synthase, O-sialoglycoprotein endopeptidase, t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEP, tRNA threonylcarbamoyladenosine biosynthesis protein OSGEP, t(6)A synthase, hOSGEP
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Recombinant Human OSGEP protein (denatured) is a Human Full Length protein, in the 1 to 335 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9NPF4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M P A V L G F E G S A N K I G V G V V R D G K V L A N P R R T Y V T P P G T G F L P G D T A R H H R A V I L D L L Q E A L T E S G L T S Q D I D C I A Y T K G P G M G A P L V S V A V V A R T V A Q L W N K P L V G V N H C I G H I E M G R L I T G A T S P T V L Y V S G G N T Q V I A Y S E H R Y R I F G E T I D I A V G N C L D R F A R V L K I S N D P S P G Y N I E Q M A K R G K K L V E L P Y T V K G M D V S F S G I L S F I E D V A H R M L A T G E C T P E D L C F S L Q E T V F A M L V E I T E R A M A H C G S Q E A L I V G G V G C N V R L Q E M M A T M C Q E R G A R L F A T D E R F C I D N G A M I A Q A G W E M F R A G H R T P L S D S G V T Q R Y R T D E V E V T W R D
- Accession
- Q9NPF4
- Protein length
- Full Length
- Predicted molecular weight
- 39 kDa
- Amino acids
- 1 to 335
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab1832238 was purified using conventional chromatography.
General info
- Function
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.
- Sequence similarities
Belongs to the KAE1 / TsaD family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OSGEP or O-sialoglycoprotein endopeptidase performs specific enzymatic functions related to protein processing. It is known for its role in the post-translational modification of glycoproteins. OSGEP has an approximate molecular mass of 43 kDa. This protein expresses in various human tissues including muscle and brain indicating its broad distribution and potential significance in different physiological contexts.
- Biological function summary
The protein acts within the KEOPS/EKC complex. This complex is essential for the formation of threonylcarbamoyladenosine (t6A) a universal tRNA modification. OSGEP plays a direct role in the synthesis of this modification which is necessary for proper protein synthesis and cellular function. The integrity of this process ensures the accurate translation of the genetic code affecting cellular homeostasis.
- Pathways
OSGEP contributes significantly to the tRNA modification and translation pathways. This protein is closely related to the tRNA threonylcarbamoyladenosine biosynthesis pathway which is critical for protein translation accuracy. It interfaces with other components of the KEOPS/EKC complex such as TP53RK and LAGE3 contributing collectively to this tRNA modification process required for effective protein synthesis.
- Associated diseases and disorders
OSGEP mutations have connections to Galloway-Mowat syndrome a rare neurogenetic disorder. This condition highlights the protein’s importance in maintaining normal cellular functions. The interaction with KEOPS/EKC complex components like TP53RK points to a critical role in neurodevelopmental processes where dysfunction can lead to complex clinical phenotypes. Understanding OSGEP-associated pathways and disease correlations is important for developing insights into therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Human OSGEP protein (denatured) (ab183238)
15% SDS-PAGE analysis of ab183238 (3μg)
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