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AB183238

Recombinant Human OSGEP protein (denatured) (His tag N-Terminus)

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Recombinant Human OSGEP protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 335 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE.

View Alternative Names

GCPL1, OSGEP, tRNA N6-adenosine threonylcarbamoyltransferase, N6-L-threonylcarbamoyladenine synthase, O-sialoglycoprotein endopeptidase, t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEP, tRNA threonylcarbamoyladenosine biosynthesis protein OSGEP, t(6)A synthase, hOSGEP

1 Images
SDS-PAGE - Recombinant Human OSGEP protein (denatured) (His tag N-Terminus) (AB183238)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human OSGEP protein (denatured) (His tag N-Terminus) (AB183238)

15% SDS-PAGE analysis of ab183238 (3μg)

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NPF4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMPAVLGFEGSANKIGVGVVRDGKVLANPRRTYVTPPGTGFLPGDTARHHRAVILDLLQEALTESGLTSQDIDCIAYTKGPGMGAPLVSVAVVARTVAQLWNKPLVGVNHCIGHIEMGRLITGATSPTVLYVSGGNTQVIAYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMDVSFSGILSFIEDVAHRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSQEALIVGGVGCNVRLQEMMATMCQERGARLFATDERFCIDNGAMIAQAGWEMFRAGHRTPLSDSGVTQRYRTDEVEVTWRD","proteinLength":"Full Length","predictedMolecularWeight":"39 kDa","actualMolecularWeight":null,"aminoAcidEnd":335,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NPF4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OSGEP or O-sialoglycoprotein endopeptidase performs specific enzymatic functions related to protein processing. It is known for its role in the post-translational modification of glycoproteins. OSGEP has an approximate molecular mass of 43 kDa. This protein expresses in various human tissues including muscle and brain indicating its broad distribution and potential significance in different physiological contexts.
Biological function summary

The protein acts within the KEOPS/EKC complex. This complex is essential for the formation of threonylcarbamoyladenosine (t6A) a universal tRNA modification. OSGEP plays a direct role in the synthesis of this modification which is necessary for proper protein synthesis and cellular function. The integrity of this process ensures the accurate translation of the genetic code affecting cellular homeostasis.

Pathways

OSGEP contributes significantly to the tRNA modification and translation pathways. This protein is closely related to the tRNA threonylcarbamoyladenosine biosynthesis pathway which is critical for protein translation accuracy. It interfaces with other components of the KEOPS/EKC complex such as TP53RK and LAGE3 contributing collectively to this tRNA modification process required for effective protein synthesis.

OSGEP mutations have connections to Galloway-Mowat syndrome a rare neurogenetic disorder. This condition highlights the protein’s importance in maintaining normal cellular functions. The interaction with KEOPS/EKC complex components like TP53RK points to a critical role in neurodevelopmental processes where dysfunction can lead to complex clinical phenotypes. Understanding OSGEP-associated pathways and disease correlations is important for developing insights into therapeutic interventions.
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Specifications

Form

Liquid

Additional notes

ab1832238 was purified using conventional chromatography.

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General info

Function

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.

Sequence similarities

Belongs to the KAE1 / TsaD family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.
See full target information OSGEP
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