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Recombinant Human Osteopontin protein (Active) is a Human Full Length protein, in the 17 to 314 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, MS, HPLC, Cell Culture

Biologically active

Yes

Actual molecular weight

33.77 kDa

Sequence

I P V K Q A D S G S S E E K Q L Y N K Y P D A V A T W L N P D P S Q K Q N L L A P Q N A V S S E E T N D F K Q E T L P S

K S N E S H D H M D D M D D E D D D D H V D S Q D S I D S N D S D D V D D T D D S H Q S D E S H H S D E S D E L V T D F

P T D L P A T E V F T P V V P T V D T Y D G R G D S V V Y G L R S K S K K F R R P D I Q Y P D A T D E D I T S H M E S E

E L N G A Y K A I P V A Q D L N A P S D W D S R G K D S Y E T S Q L D D Q S A E T H S H K Q S R L Y K R K A N D E S N E

H S D V I D S Q E L S K V S R E F H S H E F H S H E D M L V V D P K S K E E D K H L K F R I S H E L D S A S S E V N

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Cell Culture
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant Human Osteopontin protein (Active) is a Human Full Length protein, in the 17 to 314 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, MS, HPLC, Cell Culture

Biological activity

Addition of Calcein-labelled HEK-293 cells to immobilized human Osteopontin (≥1 μg) led to 16.6% specific cell adherence.

Predicted molecular weight

33.77 kDa

Amino acids

17 to 314

Sequence

I P V K Q A D S G S S E E K Q L Y N K Y P D A V A T W L N P D P S Q K Q N L L A P Q N A V S S E E T N D F K Q E T L P S

K S N E S H D H M D D M D D E D D D D H V D S Q D S I D S N D S D D V D D T D D S H Q S D E S H H S D E S D E L V T D F

P T D L P A T E V F T P V V P T V D T Y D G R G D S V V Y G L R S K S K K F R R P D I Q Y P D A T D E D I T S H M E S E

E L N G A Y K A I P V A Q D L N A P S D W D S R G K D S Y E T S Q L D D Q S A E T H S H K Q S R L Y K R K A N D E S N E

H S D V I D S Q E L S K V S R E F H S H E F H S H E D M L V V D P K S K E E D K H L K F R I S H E L D S A S S E V N

Accession
P10451-1
Protein length

Full Length

Animal free

Yes

Nature

Recombinant

Species

Human

Reconstitution

Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

Concentration
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Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Specifications

Form

Lyophilized

Additional notes

Equal or greater than 95% by HPLC

General info

Function

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Sequence similarities

Belongs to the osteopontin family.

Post-translational modifications

Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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